摘要
The preservation of pathogenic microbial strains is of great significance for basic research in microbiology and utilization of microbial resources.Appropriate preservation methods can maintain high survival rate and genetic stability of pathogenic microbial strains for a long time.This study summarizes the current status of preservation of strains,discusses the limitations and damaging factors of cryopreservation and prospects for optimization,and explores the mechanism of cryopreservation of strains.Our main objective is to provide a reference for the long-term effective preservation of microbial resources and guarantee of national biosafety.
基金
the National Science and Technology Major Project on Important Infectious Diseases Prevention and Control(2018ZX10734404)
Key technology research for standardized identification of important pathogens and establishment of relevant reference libraries and the National Natural Science Foundation of China(No.51576132,52076140).
