摘要
Osteosarcoma is a primary bone tumor that affects children and young adults.The estrogen metabolite 2-methoxyestradiol(2-ME)induces cell death in osteosarcoma cells.To determine whether 2-ME actions involve the control of protein synthesis,we studied the effect of 2-ME on eukaryotic initiation factor 4E(eIF4E)and eIF4E-binding protein 1(4E-BP1)in MG63 osteosarcoma cells.Our results show that 2-ME treatment increases the association of eIF4E with 4E-BP1 in osteosarcoma cells.Also,2-ME decreases the binding of eIF4E protein to 7-methyl-guanosine cap structure,indicating that 2-ME treatment results in the inhibition of translational initiation.These findings are further supported by the inhibition of protein synthesis in 2-ME-treated osteosarcoma cells.Taken together,our studies show that 2-ME-mediated antitumor effects in osteosarcoma cells involve the regulation of protein synthesis,and translational machinery could serve as a target in the treatment of osteosarcoma.
