消癌解毒方黄酮类成分及其给药后在大鼠体内分布

Identification of Flavonoids in Xiaoai Jiedu Decoction and Its Distribution in Rats

目的:建立超高效液相色谱-串联四极杆飞行时间质谱法(UPLC-Q-TOF-MS)检测消癌解毒方水提液中黄酮类成分的方法,同时研究大鼠灌胃给予消癌解毒方水提液后黄酮类成分在体内的分布情况。方法:采用Agilent C 18(100 mm×2.1 mm,1.8μm)反相色谱柱对各待测物进行分离,以乙腈-0.1%甲酸水溶液为流动相梯度洗脱,应用电喷雾离子源(ESI),在正、负离子模式下采集数据。结果:从消癌解毒方水提液中共鉴定出23个黄酮类化学成分;大鼠经灌胃给予消癌解毒方水提液后,6-甲氧基柚皮素(13)、柚皮素(14)、4′-羟基汉黄芩素(15)、芹菜素(16)、汉黄芩素(19)在多个生物样品中被检测发现,其中在肠、脑、胃、粪便样品中分别检测出6、6、12、10个黄酮类成分。结论:建立的方法可快速分析鉴定出消癌解毒方水提液中及给药后大鼠有关部位中黄酮类成分,为进一步阐释复方中特征化学成分及其在体内的变化分布规律提供参考。

Objective:Ultra-high performance liquid chromatography-tandem-quadrupole-time of fligh-mass spectrometry(UPLC-Q-TOF-MS)was used to identify the flavonoids in Xiaoai Jiedu Decoction(XJD)water extract and their distribution in rats after oral administration of XJD.Methods:The analytes were separated on an Agilent C 18 column(100 mm×2.1 mm,1.8μm),and eluted with a gradient of acetonitrile-water containing 0.1%formic acid.The mass spectrometer equipped with electron spray ionization(ESI)was used as detector and operated under the positive and negative ion mode.Results:23 flavonoids were isolated and identified.Among them,compounds 6-methoxynaringenin(13),naringenin(14),4′-hydroxwogonin(15),apigenin(16),wogonin(19)were detected in multiple biological samples.Among them,6,6,12,and 10 species flavonoids were detected in intestinal,brain,stomach,and stool samples,respectively.Conclusion:The liquid-mass method established in this experiment can quickly analyze and identify the flavonoids in XJD and the flavonoids in the effective parts of rats after oral administration of XJD.