白芷 白术 白附子提取物对酪氨酸酶抑制作用的比较

Comparative Study on the Inhibitory Effect of Angelicae Dahuricae Radix,Atractylodis Macrocephalae Rhizoma and Typhonii Rhizoma Extract on Tyrosinase

目的:对白芷、白术、白附子水提物和醇提物对酪氨酸酶的抑制作用进行比较。方法:应用酪氨酸酶活性抑制实验,通过测定加入中药提取物前后对生成多巴醌的影响来评估中药提取物对酪氨酸酶的抑制作用,从而对中药提取物的美白活性进行比较和评估。结果:以此方法比较了白芷、白术、白附子3种中药水提液和醇提液对酪氨酸酶的抑制作用。其中,白芷提取物对酪氨酸酶的抑制作用比白术和白附子强,白芷醇提液对酪氨酸酶的抑制率达30.77%,白芷水提液对酪氨酸酶的抑制率达20.40%,白术醇提液对酪氨酸酶的抑制率达14.71%,白术水提液对酪氨酸酶的抑制率达8.69%;白附子醇提液对酪氨酸酶的抑制率达8.36%,白附子水提液对酪氨酸酶的抑制率达3.34%。结论:为进一步揭示中药提取物美白活性成分的物质基础提供了参考。

Objective:To compare the inhibition of tyrosinase by water extract and alcohol extract of Angelicae Dahuricae Radix,Atractylodes Macrocephalae Rhizoma and Typhonii Rhizoma.Methods:Their human skin whitening activity was assessed by tyrosinase inhibition test.The inhibitory effect of Chinese medicine extract on the production of dopoquinone was evaluated by measuring the effect of dopoquinone before and after adding Chinese medicine extract.Results:This method was used to compare the inhibitory effects of three Chinese medicine water extracts and alcohol extracts on tyrosinae.Among them,the inhibitory effect of Angelicae Dahuricae Radix extract on tyrosinase was higher than that of Atractylodis Macrocephalae Rhizoma and Typhonii Rhizoma.The inhibitory rate of alcohol extract of Angelicae Dahuricae Radix on tyrosinase was 30.77%and the inhibitory rate of water extract of Angelicae Dahuricae Radix on tyrosinase was 20.40%.The inhibition rate of alcohol extract of Atractylodis Macrocephalae Rhizoma on tyrosinase was 14.71%and the inhibition rate of water extract of Atractylodis Macrocephalae Rhizoma on tyrosinase was 8.69%.The inhibition rate of alcohol extract of Typhonii Rhizoma on tyrosinase was 8.36%and the inhibition rate of water extract of Typhonii Rhizoma on tyrosinase was 3.34%.Conclusion:The study provides a reference for revealing the material basis of whitening active ingredients of traditional Chinese medicine extracts.