三个大果型番茄花药愈伤组织诱导分析

Anther Callus Induction Analysis of Three Large Fruit Tomato

本研究以3个大果型番茄杂交种F1代(‘1401’,‘1403’,‘卡莱’)的花药为试验材料,对其花药愈伤组织诱导的影响因素进行初步研究。结果表明:大果型番茄花药小孢子发育时期与花蕾长度以及花药长度呈正相关。较适番茄花蕾消毒方法为用70%酒精对番茄花蕾进行表面消毒30 s,然后再用加了0.1%吐温20的15%NaClO消毒7 min。3个供试番茄品种均能诱导产生愈伤组织,但不同番茄品种的愈伤组织诱导率不同。其中,‘1401’番茄的愈伤组织诱导率最高,为19.67%。诱导培养基中添加30 g/L蔗糖能促进愈伤组织的产生,生长素与细胞分裂素浓度比为1:2有利于愈伤组织的产生。较适愈伤组织诱导培养基为(murashige and skoog medium,MS)+0.5 mg/L IAA+1 mg/L 6-BA+30 g/L蔗糖+7 g/L琼脂粉。该研究为获得大果型番茄多倍体材料提供了技术支撑。

The anthers of three large fruit tomato F1 hybrids(’1401’,’1403’,’Kale’)were used as experimental materials to preliminarily study the influencing factors of anther callus induction.The results showed that there was a positive correlation between the microspore developmental stage and the length of flower bud or anther.The appropriate sterilizing method of tomato flower bud was to surface-sterilize the tomato flower bud with 70%alcohol for 30 s,then sterilize it with 15%NaClO with 0.1%Tween 20 for 7 min.Callus could be induced from three tested tomato varieties,but the callus induction rate of different tomato varieties was different.Among them,’1401’tomato had the highest callus induction rate(19.67%).The induction medium with 30 g/L sucrose can promote the production of callus,the ratio of auxin to cytokinin was 1:2,which was beneficial to callus production.The appropriate medium for callus induction was MS+0.5 mg/L IAA+1 mg/L 6-BA+30 g/L sucrose+7 g/L agar powder.This study provided technical support for obtaining polyploidy materials of large fruit tomato.