不同培养条件下酿酒酵母菌的转录组差异分析

Transcriptome analysis of Saccharomyces cerevisiae under different culture conditions

从组学水平分析富硒条件下酿酒酵母菌(Saccharomyces cerevisiae)内在分子机制,为酿酒酵母菌富硒研究及富硒基因的挖掘利用提供理论依据。该研究以不加硒培养的酿酒酵母菌作为对照组Kb,以加20μg/mL硒培养的酿酒酵母菌为实验组Se,利用Illumina高通量测序平台对两组进行转录组测序,通过生物信息学方法对数据进行分析处理。结果表明,转录组测序共获得6445个Unigenes,分别有1401个(21.74%)、3665个(56.87%)、5630个(87.35%)、6112个(94.83%)、6077个(94.29%)、5059个(78.49%)Unigenes被注释到GO、KEGG、COG、NR、Swiss Prot和Pfam数据库,共有6150个(95.42%)Unigenes得到注释。在GO功能注释中,共得到41个GO功能小类,在KEGG代谢通路分析时,获得了113条KEGG通路。该转录组测序数据质量高,结果覆盖面广,为酿酒酵母菌富硒基因挖掘和研究提供了一定的理论参考。

To analyze the internal molecular mechanism of Saccharomyces cerevisiae under selenium-enriched conditions from the omics level and provide a theoretical basis for the study of selenium-enriched S.cerevisiae and the mining and utilization of selenium-enriched genes,S.cerevisiae cultured without selenium was used as the control group Kb,and S.cerevisiae cultured with 20μg/mL selenium was used as the experimental group Se.Illumina high-throughput sequencing platform was used to sequence the transcriptome.Data were analyzed and processed by a variety of bioinformatics methods.The results showed that a total of 6445 Unigenes were obtained by transcriptome sequencing,including 1401(21.74%),3665(56.87%),5630(87.35%),6112(94.83%),6077(94.29%)and 5059(78.49%)Unigenes were annotated to GO,KEGG,COG,NR,Swiss Prot and Pfam databases,and a total of 6150(95.42%)Unigenes were annotated.In the GO functional annotation,a total of 41 GO functional subclasses were obtained,and 113 KEGG pathways were obtained in the KEGG metabolic pathway analysis.The high-quality transcriptome sequencing data and wide coverage provide a certain theoretical reference for selenium-enriched gene mining and research of S.cerevisiae.