应用共聚焦拉曼光谱法对烟酰胺在人体皮肤角质层渗透性的研究

Penetration research of niacinamide in stratum corneum of human skin by confocal Raman spectroscopy

通过对受试者使用含烟酰胺成分的产品不同时间皮肤拉曼光谱的分析,检测烟酰胺在皮肤角质层中的渗透深度及渗透量,对烟酰胺在皮肤角质层内的透皮吸收性能进行评价,验证共聚焦拉曼光谱技术在成分透皮吸收领域应用的可行性。结果表明,烟酰胺的拉曼特征峰在不同基质中具有稳定性,含3%烟酰胺的膏霜涂抹于皮肤后,烟酰胺在皮肤中渗透量随着时间延长而增加,2 h后含量(AUC)约55520.06。从拉曼光谱及渗透量变化的定性和定量角度表明:含3%烟酰胺的膏霜涂抹于皮肤2 h后,烟酰胺渗透深度约为16μm。共聚焦拉曼光谱法在烟酰胺的渗透性研究中得到了有效的应用,其研究方法可为化妆品、药品等成分的经皮吸收提供方法参考。

Analyzing and detecting the penetration depth and content of niacinamide in SC(stratum corneum)by skin Raman spectroscopy before and after using products which contained niacinamide was performed to evaluate the penetration ability of niacinamide and validation the feasibility of confocal Raman spectroscopy in transdermal absorption research.The results show that the characteristic peaks in Raman spectrum of niacinamide are stable in different substrates.After the cream containing 3%niacinamide is applied to the skin,the penetration content of niacinamide in SC increases with time and the content(AUC)of niacinamide in SC after 2 h is about 55520.06.From the qualitative and quantitative perspectives of changes in Raman spectrum and penetration content,it shows that after applying cream with 3%nicotinamide to the skin for 2 h,the penetration depth of nicotinamide is about 16μm.Confocal Raman spectroscopy is effectively applied in the penetration ability study of niacinamide in SC,and the research method can be a reference for the penetration of cosmetics,pharmaceuticals and other ingredients.