内生枯草芽孢杆菌JL-B16生物学特性研究

The Biological Characteristics of Endophytic Bacillus subtilis JL-B16

对枯草芽孢杆菌JL-B16进行平板培养,并对菌体进行革兰氏染色、芽孢染色和荚膜染色,获得枯草芽孢杆菌JL-B16的培养特征和形态特征;采用温度梯度和pH梯度法测定枯草芽孢杆菌JL-B16的最适培养条件,绘制其40 h内的生长曲线,并对其生理生化指标进行测定,结果表明,枯草芽孢杆菌JL-B16菌落为黄色,菌落边缘不平整,菌体呈杆状,革兰氏阳性,有芽孢,无荚膜;枯草芽孢杆菌JL-B16最适培养温度为25℃,最佳生长pH为7.0,适宜生长pH 6.0~8.0。对枯草芽孢杆菌JL-B16进行生物学特性及生理生化指标的测定,为后续系统研究枯草芽孢杆菌JL-B16奠定了基础。

The culture characteristics and morphology characteristics of Bacillus subtilis JL-B16 were obtained by plate culture,Gram staining,spore staining and capsule staining.The optimum culture conditions of B.subtilis JL-B16 were determined by temperature gradient and pH gradient method,the growth curves of B.subtilis JL-B16 were plotted within 40 hours,and their physiological and biochemical indexes were determined.The result showed that the colony color of B.subtilis JL-B16 was yellow,the colony edge was uneven,the bacteria were rod-shaped,Gram-positive,there were spores and no capsule;the optimum culture temperature of B.subtilis JL-B16 was 25℃,the optimum growth pH was 7.0,and the growth range pH was 6.0-8.0.The biological characteristics and physiological and biochemical indexes of B.subtilis JL-B16 were determined,which laid the foundation for the subsequent systematic study of B.subtilis JL-B16.