摘要
血管破裂时,血液系统参与凝血反应,起到止血作用。当植入材料与血液接触时会导致凝血级联响应,因此生物材料的血液相容性至关重要。基于纳米材料的独特性能,本研究中,壳聚糖(CS)在交联剂三聚磷酸钠(TPP)的作用下自组装形成CS-TPP胶束,通过静电相互作用在CS-TPP胶束表面接枝牛血清白蛋白(BSA),使其在CS-TPP胶束表面形成一层蛋白膜,研究其血液相容性。通过调整投料比及反应条件,可实现对胶束尺寸的控制。材料学表征结果显示,CS-TPP-BSA胶束的尺寸均一,形态稳定,分散性良好。随后将CS-TPP-BSA胶束通过多巴胺接枝于材料表面,血液试验证实该胶束涂层能够显著提高血液接触材料的血液相容性。该研究为血液接触生物材料的表面改性提供了一种新的手段。
As blood system itself is able to start the self-protective coagulation process to stop bleeding immediately in damaged vessel,blood-contacting materials will severely increase the risk of initiating a coagulation cascade.Therefore,it's vital to ensure the good blood compatibility of blood-contacting materials.In this paper,based on the unique properties of nanomaterials,a chitosan micelle was developed(CS-TPP)firstly by cross-linking agent sodium tripolyphosphate(TPP)-assisted self-assembly.Afterwards,bovine serum albumin was grafted on micelle surface via electrostatic interaction to form CS-TPP-BSA micelles for blood compatibility evaluation.The material characterization results showed that CS-TPP-BSA micelles were uniform,stable and well-dispersed.Besides,micelle size could be regulated by optimizing feed ratios and reaction condition.Micelles were then coated on material surface via dopamine,and blood testing indicated that CS-TPP-BSA coating could significantly enhance the blood compatibility,which provided a new surface modification method for blood-contacting materials.
作者
王倩
李莉
王焕然
卞齐豪
翁亚军
陈俊英
WANG Qian;LI Li;WANG Huanran;BIAN Qihao;WENG Yajun;CHEN Junying(School of Life Sciences and Engineering, Southweast Jiaotong University, Chengdu 610031, China;School of Materials Sciences and Engineering,Southweast Jiaotong University, Chengdu 610031, China)
出处
《功能材料》
EI
CAS
CSCD
北大核心
2021年第1期1214-1220,共7页
Journal of Functional Materials
基金
国家自然科学基金资助项目(31870955,31470921)
国家重点研发资助项目(SQ2019YFC110049)。
关键词
壳聚糖
牛血清白蛋白
自组装
胶束
血液相容性
chitosan
bovine serum albumin
self-assembly
micelles
blood compatibility