摘要
以番石榴为原料,通过超声波辅助提取番石榴黄酮,采用AB-8大孔树脂进行分离纯化,利用溶剂挥发自组装方法制备玉米醇溶蛋白-番石榴黄酮复合纳米颗粒(zein-guava flavonoid composite nanoparticles,ZGF)。通过扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱和差示扫描量热法等技术对样品的形貌和特性进行表征。通过四甲基偶氮唑蓝法研究番石榴黄酮和ZGF对细胞活力的影响,通过DCFH-DA活性氧荧光探针法研究ZGF对细胞产生活性氧的影响,并通过1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率的测定评价ZGF的抗氧化活性,探究ZGF样品在人工胃液中的释放规律。结果表明,分离纯化后的番石榴黄酮质量分数为67.0%;玉米醇溶蛋白与番石榴黄酮质量比为16∶1时,包埋率达到最大值,为93.29%;制备的ZGF为直径500 nm左右的球形结构;ZGF在0.5~5 mg/mL范围内对HepG2细胞的活力基本没有影响;同时,ZGF能有效抑制H2O2诱导HepG2细胞产生的氧化损伤,并且呈浓度依赖关系;当ZGF的质量浓度在416.67μg/mL时,DPPH自由基的清除率高达86.02%。此外,在1~6 h内,ZGF在人工胃液中的累积释放率随时间延长而增加,6 h时的累积释放率为64.11%,表明黄酮释放效果良好。研究结果可为番石榴黄酮的综合利用提供理论参考。
Flavonoids from guava fruit were obtained though ultrasonic-assisted extraction and were purified using an AB-8 macroporous resin column before being used to prepare zein-guava flavonoid composite nanoparticles(ZGF)by a solvent evaporation-induced self-assembly method.Scanning electron microscopy(SEM),transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FTIR)and differential scanning calorimetry(DSC)were used to explore the morphology and characteristics of the as-prepared samples.In addition,the cytotoxicity of the flavonoids and the composite nanoparticles was studied by the methyl thiazolyl tetrazolium method and the impact of ZGF on cellular reactive oxygen species(ROS)production was evaluated using the fluorescent probe DCFH-DA.The antioxidant activity of ZGF was evaluated by measuring the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging activity.The release behavior of ZGF in artificial gastric juice was investigated.The results showed that the purity of the purified flavonoids was 67.0%.The maximum encapsulation efficiency of 93.29%was obtained when the mass ratio of zein to flavonoids was 16:1.The size of the prepared nanoparticles was approximately 500 nm with a spherical structure.ZFG had no obvious cytotoxicity on HepG2 cells in the concentration range of 0.5–5 mg/mL.Moreover,ZFG could protect HepG2 cells from oxidative damage induced by H2O2 in a concentration-dependent manner and could scavenge 86.02%of DPPH radicals at a concentration of 416.67μg/mL.Furthermore,the cumulative release rate of ZGF in artificial gastric juice was increased from 1 to 6 h of digestion reaching 64.11%,suggesting good release performance.This study provides a theoretical reference for the comprehensive utilization of guava flavonoids.
作者
周浓
余志良
李承勇
ZHOU Nong;YU Zhiliang;LI Chengyong(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China;Shenzhen Institute of Guangdong Ocean University,Shenzhen 518018,China;College of Chemistry and Environment,Guangdong Ocean University,Zhanjiang 524088,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2021年第3期186-193,共8页
Food Science
基金
湛江市科技专项竞争性分配项目(2017A02022)。