亚致死浓度氟啶虫胺腈对桃蚜体内能量物质的影响

Effects on the Energy Substances of Myzus persicae by the Sublethal Concentration of Sulfoxaflor

[目的]明确氟啶虫胺腈亚致死浓度对桃蚜体内能量物质的影响,旨在为氟啶虫胺腈对靶标害虫的解毒代谢机理提供更多的理论参考。[方法]通过GC-MS分析法,测定氟啶虫胺腈亚致死浓度(LC10和LC30)胁迫下,桃蚜体内脂肪酸、氨基酸及可溶性糖的组成及含量变化。[结果]氟啶虫胺腈亚致死浓度作用下,桃蚜体内的总脂肪酸含量显著降低,除了LC10处理组的豆蔻油酸,LC30处理组的花生烯酸和神经酸,药剂处理组各脂肪酸含量均显著低于对照处理组;总氨基酸含量LC10处理组显著低于对照处理组,LC30处理组显著高于对照处理组,与对照处理相比,LC10处理组未检测到蛋氨酸,而LC30处理组除了检测到对照组中所有游离氨基酸外,另外检测到谷氨酸;与对照组相比,药剂处理组总可溶性糖含量均有所增加,但LC10处理组与对照组无显著性差异,LC30处理组显著高于对照处理组。[结论]氟啶虫胺腈亚致死浓度作用下,桃蚜体内的脂肪酸、氨基酸及糖类组成及含量均会发生不同程度的变化,上述能量物质在一定程度上参与了桃蚜对氟啶虫胺腈的解毒代谢过程,但具体的解毒代谢过程有待进一步研究。

[Aims] This study aims to clarify the effects of sulfoxaflor at sublethal concentrations on the energy substances in Myzus persicae, and to provide more theoretical references of detoxification and metabolism mechanism of sulfoxaflor to target pests. [Methods] The changes of fatty acids, amino acids and the composition and content of soluble sugars in M. persicae treated with sulfoxaflor at sublethal concentrations(LC10 and LC30) were determined by GC-MS. [Results] Under the stress of sulfoxaflor at sublethal concentrations, the total fatty acids content significantly decreased in M. persicae, except the treatment with sulfoxaflor at LC10 for cardamom oleic acids and LC30 for arachidonic acids and nervonic acids. The fatty acids content of treatment with sulfoxaflor at sublethal concentrations were all notably lower than that of control. After being exposed to sulfoxaflor at LC10, the total amino acids content was all remarkably less than that of control group, while the treatment with sulfoxaflor at LC30 was significantly higher than the control group. Compared with the control group, methionine was not detected in the treatment with sulfoxaflor at LC10, while the treatment with sulfoxaflor at LC30 was not only detected all amino acids but also detected glutamic acids;compared with the control group, all the content of soluble sugar of the treatments with chemical significantly increased, however, there were no obvious differences between treatment with sulfoxaflor at LC10 and control, the treatment with sulfoxaflor at LC30 indicated greatly higher content of soluble sugar than the other two treatments.[Conclusions] After being treated with sulfoxaflor at sublethal concentrations, the changes of the fatty acids, amino acids and the composition and content of carbohydrates all happened in different degrees, indicating these energy substances mentioned above participate in the detoxification metabolism process of M. persicae to some extent, but the specific detoxification metabolism process needs to be further studied.