PD-L1基因多态性对食管癌患者临床病理特征及临床预后质量的影响

Influence of PD-L1 gene polymorphism on clinicopathological characteristics and clinical prognosis quality of patients with esophageal cancer

目的:探讨食管癌患者中程序性死亡-配体1(programmed death-ligand 1,PD-L1)基因多态性对病理特征以及临床预后质量的影响。方法:选取本院诊治的食管癌患者86例作为观察组,同期选取100例健康体检患者作为对照组,采用直接测序法以及PCR-SSCP法测定PD-L1基因不同位点rs2890658、rs17718883、rs2297136以及rs4143185的单核苷酸多态性,分析PD-L1基因多态性对食管癌病理特征以及预后的影响。结果:观察组最终完成研究84例,对照组纳入研究99例。观察组与对照组在PD-L1不同基因位点rs17718883、rs2890658、rs2297136基因位点对比有差异(P<0.05)。PD-L1基因位点rs17718883与食管癌病理类型、分化程度有关,差异具有统计学意义(P<0.05),rs2890658与食管癌分化程度有关,差异具有统计学意义(P<0.05)。log-rank检验显示,食管癌患者rs17718883位点基因型与预后生存有关,差异具有统计学意义(P<0.05)。Cox比例风险模型分析显示,年龄、分化程度、淋巴结转移以及rs17718883基因型是食管癌患者预后的独立因素,差异具有统计学意义(P<0.05)。结论:PD-L1基因rs17718883、rs2890658位点单核苷酸多态性对食管癌患者临床病理特征有影响,PD-L1基因rs17718883位点单核苷酸多态性对临床预后质量有影响。

Objective:To investigate the influence of programmed death-ligand 1(PD-L1)gene polymorphism on pathological characteristics and clinical prognosis quality in patients with esophageal cancer.Methods:A total of 86 patients with esophageal cancer treated in our hospital from January 2014 to January 2017 were selected as the observation group,and 100 healthy patients were selected as the control group during the same period.The single nucleotide polymorphisms of rs2890658,rs17718883,rs2297136 and rs4143185 at different sites were determined with sequencing method and PCR-SSCP method in order to explore the influences of PD-L1 gene polymorphism on pathological features and prognosis of esophageal cancer.Results:There were significant differences in the PD-L1 different gene loci rs17718883,rs2890658,rs2297136(P<0.05)between the observation group(n=84 cases)and the control group(n=99 cases)(P<0.05).The PD-L1 locus rs17718883 was significantly related to the pathological type and degree of differentiation of esophageal cancer,rs2890658 was significantly related to the degree of differentiation of esophageal cancer(P<0.05).Log-rank test showed that the genotype of rs17718883 in patients with esophageal cancer was related to prognostic survival,and the difference was statistically significant(P<0.05).Cox proportional hazards model analysis showed that age,degree of differentiation,lymph node metastasis,and rs17718883 genotype were independent risk factors for the prognosis of patients with esophageal cancer,and the difference was statistically significant(P<0.05).Conclusion:The polymorphisms of rs17718883 and rs2890658 loci of PD-L1 gene have an impact on the clinicopathological characteristics of patients with esophageal cancer,while the polymorphisms of rs17718883 locus of PD-L1 gene have an impact on the clinical prognosis quality.