摘要
以分离后的单壁碳纳米管为载药工具,制备了一种(6,5)单壁碳纳米管-盐酸阿霉素-人血清白蛋白-叶酸((6,5)SWCNT-Dox-HSA-FA)四元靶向载药系统。首先通过反应制备得到三元复合物Dox-HSA-FA,并采用双水相萃取法分离得到(6,5)SWCNT;然后采用超声作用将(6,5)SWCNT分散至Dox-HSA-FA中制备得到(6,5)SWCNT-Dox-HSA-FA;最后采用Hep G2细胞对四元复合物进行细胞毒性评价。结果表明,Dox-HSA-FA和(6,5)SWCNT-Dox-HSAFA的半数抑制浓度(IC_(50))分别为27.3μg/mL和25.7μg/mL,表明四元复合物可以提高盐酸阿霉素的靶向性。
A drug delivery system was designed in this paper.The system was composed of(6,5)chiral singlewalled carbon nanotube((6,5)SWCNT),doxorubicin hydrochloride(Dox),human serum albumin(HSA),and folic acid(FA).Firstly,the Dox-HSA-FA was prepared and(6,5)SWCNT was isolated by aqueous two-phase extraction.Then,(6,5)SWCNT-Dox-HSA-FA was dispersed into Dox-HSA-FA by ultrasonication.Finally,cytotoxicity evaluation of the complex was performed using Hep G2 cells.The results showed that the IC50 of Dox-HSA-FA and(6,5)SWCNTDox-HSA-FA was 27.3μg/mL and 25.7μg/mL,respectively.The results indicated that the targeting effect of Dox was enhanced in the(6,5)SWCNT-Dox-HSA-FA complex.
作者
仲文博
张敏
章弘扬
王月荣
胡坪
ZHONG Wenbo;ZHANG Min;ZHANG Hongyang;WANG Yuerong;HU Ping(Shanghai Key Laboratory of New Drug Design,School of Pharmacy;Shanghai Key Laboratory of Functional Materials Chemistry,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China)
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第1期41-47,共7页
Journal of East China University of Science and Technology
基金
国家自然科学基金(81573397)。
关键词
单壁碳纳米管
盐酸阿霉素
人血清白蛋白
叶酸
靶向
single-walled carbon nanotubes
doxorubicin hydrochloride
human serum albumin
folic acid
targeting
