基于16S rDNA测序的黄芪总多糖对SD大鼠肠道微生物多样性的影响

Based on Sequencing of 16Sr DNA Gene Elaborate Effect of Total Polysaccharides of Astraglus Membranaceus on Intestinal Microbial Diversity in SD Rats

目的:研究黄芪总多糖(Total Polysaccharides of Astraglus Membranaceus, PSA)对SD大鼠肠道微生物多样性的影响。方法:SPF级SD大鼠随机分为对照组,黄芪总多糖低剂量组(100mg/kg)和高剂量组(400mg/kg),连续灌胃30d。于第31d取粪便进行16S rDNA高通量测序和微生物共发生关系网络分析。结果:与对照组相比高剂量组显著上调12个属,下调7个属;低剂量组显著上调16个属,下调8个属。PSA影响肠道菌群间的相互作用,起主导作用的为厚壁门、放线菌门、拟杆菌门、柔膜菌门、变形菌门和迷踪菌门。结论:PSA能改变大鼠肠道微生物多样性,促进丁酸产盐菌属、魏斯氏菌属、理研菌属、乳酸杆菌属等有益菌的增殖,抑制肠杆菌属、拟杆菌属等有害菌的生长,以厚壁门为核心的6个门微生物在菌群间相互作用中起着主导作用。

Objective: To study the effect of Total Polysaccharides of Astraglus Membranaceus(PSA) on intestinal microbial diversity in normal SD rats. Methods: SD rats were randomly divided into controlled group, PSA low-dose group(100 mg/kg) and high-dose group 400 mg/kg, and gavaged continuously for 30 days. On 31st day, the faeces were collected and sequenced by 16 S rDNA high-throughput sequencing technology. The network analysis of microbial co-occurrence relationship was used to find the effects of microbial environment which was brewed by PSA on microbial adaptability. Results: Compared with the controlled group, there were 12 genus including Weissella were significantly up-regulated in the high-dose group, and 7 genus including Enterorhabdus were significantly down-regulated. In the low-dose group, there were 16 genus including Rikenella were significantly up-regulated, and 8 genus including Alistipes were significantly down-regulated. Network analysis of microbial co-occurrence relationship showed that after entering the digestive system, PSA may affect the intestinal microbial diversity through influences on interactions between intestinal flora. In these interactions, the leading bacteria were the following 6 phylum: Firmicutes, Actinobacteria, Bacteroidetes, Tenericutes, Proteobacteria, Elusimicrobia. Conclusion: PSA can change the intestinal microbial diversity of SD rats. It can promote the growth of Anaerostipes, Weissella, Rikenella, Lactobacillus and other beneficial bacteria, and inhibit the growth of harmful bacteria such as Enterorhabdus and Bacteroides. Six phylum microorganisms, with Firmicutes being the most dominant, play a leading role in the interaction among the microorganisms.