红花多糖对胃癌BGC-823细胞增殖抑制作用的研究

The Pilot Study on the Inhibition of Safflower Polysaccharide to Human Gastric Carcinoma Cell Line BGC-823

目的:探讨红花多糖对人胃癌BGC-823细胞增殖抑制效应。方法:不同浓度的红花多糖作用于人胃癌BGC-823细胞,采用MTT法检测药物对BGC-823细胞增殖的抑制作用、倒置显微镜观察BGC-823细胞的形态改变及Hoechst 33342染色观察胃癌细胞凋亡的情况。结果:红花多糖对胃癌BGC-823细胞增殖具有明显的抑制作用,且呈一定的时间与剂量依赖;光镜下可见凋亡细胞的形态学特征性改变。结论:红花多糖能抑制人胃癌BGC-823细胞增殖,同时可诱导胃癌细胞凋亡。

Objective:To observe the effect of safflower polysaccharide on the cell proliferation and apoptosis of human gastric carcinoma cell line of BGC-823.Methods:BGC-823 cells were processed with different concentrations of safflower polysaccharide during the same period.Cell viability was measured by MTT assay.Morphological changes of the cells were observed under invert microscope after staining with Hoechst 33342.Results:Safflower polysaccharide could inhibit the growth of BGC-823 cell lines in dosage and time dependant manner.Cells exhibited apoptosis in morphology.Conclusion Safflower polysaccharide could inhibit the proliferation and induce the apoptosis of gastric cancer cell lines of BGC-823.