丝素蛋白/磷酸八钙复合材料生物界面的蛋白质吸附和细胞响应

Protein adsorption and cell response on bio-interfaces of silk fibroin/octacalcium phosphate composites

为进一步了解仿生材料结构与功能的关系,为体内骨整合设计一个有利的种植微环境,通过电化学沉积技术调控电解液中丝素蛋白(SF)的质量浓度,制备了具有纳微米多级结构的丝素蛋白/磷酸八钙(SF/OCP)复合涂层。研究了丝素蛋白质量浓度对SF/OCP复合涂层的表面形貌、力学性能、蛋白质吸附以及细胞增殖行为的影响。结果表明:随电解液中丝素蛋白质量浓度的增加,涂层表面孔洞直径由(19.96±6.96)μm减小至(1.56±0.22)μm,OCP晶体宽度减小至纳米级别((26.84±8.2)nm);与纯OCP涂层相比,SF/OCP复合涂层(SF质量浓度为1.0 mg/mL)的弹性模量和硬度分别增加了约1.5和4.3倍;SF/OCP复合涂层选择性地增强了纤连蛋白(Fn)的吸附,经7 d的细胞培养后,SF/OCP复合涂层(SF质量浓度为1.0 mg/mL)表面的细胞活力是纯OCP涂层上的1.28倍。

To further understand the structure-function relationships for biomimetic materials for the purpose of designing a favorable implant microenvironment for osseointegration in vivo,the nano/micro hierarchical structure of silk fibroin(SF)/octacalcium phosphate(OCP)composite coating was constructed by controlling simply the SF concentration of the electrolyte during the electrochemical deposition process.The influence of SF concentration on surface morphology,mechanical property,protein adsorption and cell proliferation were studied.The results show that the pore diameter of the coating surface decreases from(19.96±6.96)μm to(1.56±0.22)μm and the crystal width decreases to the nano-scale with SF concentration increasing in electrolyte.Comparing the pure OCP coating,the elastic modulus and hardness of the SF/OCP composite coating(1.0 mg/mL SF)increase approximately 1.5 times and 4.3 times,respectively.The SF/OCP composite coating selectively enhances fibronectin(Fn)adsorption,and the cell viability on the SF/OCP composite coating(1.0 mg/mL SF)is 1.28 times that on the pure OCP coating after culturing for 7 days.