基于海藻酸钠/磷虾蛋白的支架材料制备及其性能

Preparation and properties of sodium alginate/krill protein scaffold materials

为提高海藻酸钠基支架材料的强度和生物相容性,以海藻酸钠(SA)和磷虾蛋白(AKP)构成聚电解质复合体系,通过低温诱导相分离-化学交联法制备SA/AKP支架材料,比较不同质量分数SA/AKP溶液制备的支架材料的结构形态、力学性能、孔隙率、透气性、吸水性及生物相容性。结果表明:在SA/AKP溶液质量分数为4%时,支架孔径在20~96μm之间,孔径大小均一,形状规整;支架材料的孔隙率、透气性、液体吸收性随SA/AKP溶液质量分数增加而降低,断裂强度和断裂伸长率随SA/AKP溶液质量分数增加而增加,在质量分数为4%时,支架材料的孔隙率为86.4%,透气率为58.9%,且具有较高的强度与伸长;支架材料的细胞毒性等级为0级,具有优异的生物安全性。

In order to improve the strength and bio-compatibility of sodium alginate based scaffold materials made from polyelectrolyte composite system with sodium alginate(SA)and krill protein(AKP),SA/AKP scaffold materials were prepared by inducing phase separation-chemical cross-linking method at low-temperature.The structural morphology,mechanical properties,porosity,air permeability,water absorption and bio-compatibility of different concentrations of SA/AKP solutions were compared.The results show that when the concentration of SA/AKP sample is 4%,the pore size of the stent is between 20 and 96μm with uniform and regular shape.The porosity,and absorptive characteristics of liquid and air permeability of the tissue scaffold decreased with the increasing concentration of SA/AKP solutions,and the fracture strength and elongation of the scaffold increased with the increasing concentration of SA/AKP solutions.When the concentration is 4%,the porosity of the scaffold material is 86.4%,the air permeability is 58.9%,while offering high strength and elongation.The cell toxicity grade of the scaffold is 0,indicating excellent biological safety.