摘要
为了探究DHA脂质体对脂代谢的调节作用。首先利用游离脂肪酸诱导HepG2细胞,DHA脂质体干预后检测甘油三酯(TG),胆固醇(TC)含量,RT-PCR检测脂肪酸合成酶(FAS),固醇调节元件结合蛋白1(SREBP-1)的m RNA表达。其次将雄性C57BL/6J小鼠(6周龄)随机分为对照组(C)、模型组(M)、DHA脂乳剂组(O-DHA)、DHA脂质体组(L-DHA)。12周后,检测脂肪酸合成酶(FAS),肉碱棕榈酰转移酶1(CPT-1)蛋白表达量以及脂肪酸合成酶(FAS),苹果酸酶(ME),肉碱棕榈酰转移酶1(CPT-1),脂蛋白酯酶(LPL)的酶活力。结果表明DHA脂质体可显著降低HepG2细胞的TC,TG含量(p<0.05),分别降低了62.91%和48.73%,且显著降低了FAS(48.62%),SREBP-1(38.53%)的m RNA相对表达量(p<0.05)。动物实验结果表明DHA脂质体显著降低了高脂饮食小鼠附睾脂肪含量(14.26%)(p<0.05),且显著降低了高脂饮食小鼠FAS(74.29%)蛋白表达量(p<0.05),显著升高了CPT-1(约1.70倍)的蛋白表达量(p<0.05)。DHA脂质体还可显著抑制FAS,ME酶活力(p<0.05),分别降低了30.33%和11.79%。显著促进了CPT-1,LPL酶活力(p<0.05),分别升高了10.08%和15.12%。本研究表明DHA脂质体可改善高脂饮食诱导的脂代谢紊乱,为深海鱼油的开发利用提供理论依据。
To explore the effects of DHA liposomes on lipid metabolism,HepG2 cells were induced by free fatty acids.After the intervention of DHA liposomes,the contents of TG and TC were detected.The m RNA expression of Fatty acid synthase(FAS)and Sterol regulatory element binding protein 1(SREBP-1)were detected by RT-PCR.Then male C57 BL/6 J mice(6 weeks old)were housed and randomly devided into control group(C),model group(M),TG-DHA emulsion(O-DHA),TG-DHA liposome(L-DHA).After 12 weeks,the protein expression levels of FAS,CPT-1 were determined by immunohistochemistry,the enzyme activity of Fatty acid synthase(FAS),malic enzyme(ME),carnitine palmitoyltransferase 1(CPT-1),lipoprotein esterase(LPL)were detected using ELISA.The results showed that DHA liposomes significantly reduced the TC and TG contents(p<0.05)by 62.91%and 48.73%respectively;significantly reduced the m RNA expression levels of FAS(48.62%)and SREBP-1(38.53%)(p<0.05).The results of animal experiments showed that DHA liposomes significantly reduced theepididymal fat content(14.26%)of high-fat diet mice(p<0.05);significantly reduced the protein expression levels of FAS(74.29%)(p<0.05)and increased the protein expression levels of CPT-1(1.70 folds)(p<0.05).DHA liposomes could significantly inhibited the activities of FAS,ME(p<0.05),reduced by 30.33%and 11.79%respectively;and promoted the activities of CPT-1,LPL(p<0.05),increased by 10.08%and 15.12%respectively.This study showed that DHA liposomes can improve lipid metabolism disorder induced by high-fat diet,and provide theoretical basis for the development and utilization of deep-sea fish oil.
作者
杨瑞利
卢娜
张慧
周赛楠
张青
薛长湖
唐庆娟
YANG Rui-li;LU Na;ZHANG Hui;ZHOU Sai-nan;ZHANG Qing;XUE Chang-hu;TANG Qing-juan(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Drugs and Bioproducts,Pilot National Laboratory for Marine Science and Technology,Qingdao 266237,China)
出处
《现代食品科技》
EI
CAS
北大核心
2021年第2期11-20,共10页
Modern Food Science and Technology
基金
国家重点研发计划项目(2018YFC0311201,2019YFC1604605)
山东省重点研发计划项目(2019GHY112058)。