电刺激大鼠右侧尾壳核诱导皮层脑电高频振荡的特征

The properties of the power spectrum of neocortical high frequency oscillations induced by acute tetanization of the right caudate putamen nucleus

目的探讨电刺激大鼠右侧尾壳核诱导皮层脑电高频振荡(HFO)功率谱特征。方法实验共用雄性SD大鼠56只,体质量150~250 g,氨基甲酸乙酯麻醉下行气管插管和开颅术。将一根双极不锈钢同芯刺激电极植于右侧尾壳核,重复施加电刺激(60 Hz,2 s,0.4~0.6 mA);将两个不锈钢螺丝钉旋入双侧皮层的颅骨内,一个不锈钢螺丝钉电极旋入小脑皮层表面的颅骨内,记录双侧皮层脑电,分析脑电HFO的功率谱特征。结果重复施加电刺激诱导出现持续时间长达近10 ms的HFO,其电活动模式呈现逐渐增大和减小的变化趋势;从功率谱上分析,显示其带宽范围为80~450 Hz,且施加刺激对侧HFO持续时间延长、振幅增强,潜伏期缩短。结论电刺激大鼠右侧尾壳核可以诱导出现双侧皮层HFO,对侧易感,形成尾壳核-皮层癫痫网络。

Objective To study the electrophysiological properties of the power spectrum of neocortical high frequency oscillations(HFOs)induced by acute tetanization(60 Hz,2 s,0.4~0.6 mA)of the right caudate putamen nucleus(ATRC).Methods Experiments were performed on 56 male rats,weighed 150~250 g.Rats were anaesthetized with 10% urethane(1g/kg,ip)endotracheally intubated and monunted in a stereotaxic apparatus.Three stainless screws were fixed on cranial bones were used for EEG recording of the surface of bilateral neocoties and one reference point on cranial bones over the surface of cerebellar cortex.Results 80~450 Hz oscillation patterned in waxing and waning;The ripple duration time was about HFO with10 ms.The contralateral oscillation became much stronger and the latent period was shortened.Conclusion ATRC can induce HFOs reflecting a Cpu-neocortical epilepsy network reorganization.