饲粮添加地衣芽孢杆菌对4~7月龄黑毛驴生长性能、血清生化指标及直肠菌群多样性的影响

Effects of Dietary Bacillus licheniformis on Growth Performance,Serum Biochemical Indexes and Rectum Content Bacteria Diversity of Black Donkeys Aged from 4 to 7 Months

本试验旨在研究饲粮添加地衣芽孢杆菌对4~7月龄黑毛驴生长性能、血清生化指标及直肠菌群多样性的影响。选取4~7月龄体况良好的黑毛驴10只,随机分为2组,每组5个重复,每个重复1头。对照组饲喂基础饲粮,试验组在基础饲粮中添加1×109 CFU/g地衣芽孢杆菌。预试期7 d,正试期60 d。结果表明:与对照组相比,1)饲粮添加地衣芽孢杆菌对黑毛驴总增重、平均日增重、干物质采食量和料重比均无显著影响(P>0.05);2)饲粮添加地衣芽孢杆菌显著降低了血清白蛋白含量(P<0.05),显著提高了血清免疫球蛋白G含量(P<0.05),对其他各项血清生化指标没有显著影响(P>0.05);3)饲粮添加地衣芽孢杆菌对黑毛驴直肠内容物菌群α多样性无显著影响(P>0.05),提高了直肠内容物中拟杆菌门和螺旋体门相对丰度。主坐标分析和Adonis分析结果显示,饲粮添加地衣芽孢杆菌对黑毛驴直肠内容物细菌组成无显著影响(P>0.05)。由此可见,饲粮添加地衣芽孢杆菌并未显著提高黑毛驴的生长性能和显著影响黑毛驴直肠细菌组成。

The aim of this study was to investigate the effects of dietary Bacillus licheniformis on growth performance,serum biochemical indexes and rectum content bacteria diversity of black donkeys aged from 4 to 7 months.Ten healthy black donkeys aged from 4 to 7 months were selected and randomly divided into 2 groups with 5 replicates per group and 1 donkey per replicate.Donkeys in the control group were fed a basal diet,and others in the experimental group were fed the basal diet supplemented with 1×109 CFU/g Bacillus licheniformis.The pre-experimental period lasted for 7 days,and the experimental period lasted for 60 days.The results showed as follows:compared to the control group,1)dietary Bacillus licheniformis had no significant effects on the total weight gain,average daily gain,dry matter intake and feed to gain ratio of black donkeys(P>0.05);2)dietary Bacillus licheniformis significantly increased the serum albumin content(P<0.05),significantly increased the serum immunoglobulin G content(P<0.05),but had no significant effects on other serum biochemical indexes(P>0.05);3)dietary Bacillus licheniformis had no significant effects on the bacteriaαdiversity in rectum contents of black donkeys(P>0.05),and increased the relative abundance of Bacteroidetes and Spirochaetes in rectum contents(P<0.05).Principal coordinate analysis and Adonis analysis results showed that dietary Bacillus licheniformis had no significant effects on the bacteria composition in rectum contents of black donkeys.In conclusion,dietary Bacillus licheniformis do not significantly improve the growth performance of black donkeys,and also do not affect the rectal bacterial composition of black donkeys.