装载雷公藤甲素的金属有机骨架材料ZIF-8的制备、表征及优化研究

Preparation,characterization and optimization of ZIF-8,a metal organic framework material loaded with triptolide

目的优选金属有机骨架材料二甲基咪唑锌盐(ZIF-8)的制备方法,并考察其用于装载雷公藤甲素的工艺条件。方法以六水合硝酸锌和2-甲基咪唑为载体材料,设计单因素试验,考察物理搅拌法、超声法及水热合成法3种制备工艺,并用X-射线粉末衍射(XRD)等方法对材料进行表征。设计正交试验,以载药量及包封率为评价指标,确定雷公藤甲素的最佳载药工艺。结果利用物理搅拌法制备的ZIF-8晶体较优且稳定,比表面积最大为1622.38 m2·g-1。最佳载药工艺为药载比5∶12,载药浓度6 mg·mL-1,载药时间3 d。3批验证数据表明,雷公藤甲素载药量与包封率分别为(49.87±1.26)%和(70.66±1.26)%。结论采用物理搅拌法能成功制得ZIF-8,其用于装载雷公藤甲素具有较高的载药量和包封率。该方法操作简便,工艺稳定。

Objective To optimize the preparation of metal organic framework material ZIF-8,and investigate the process for triptolide loading.Methods Zinc nitrate hexahydrate and 2-methylimidazole were used as carrier materials,and single factor experiment was conducted to investigate 3 preparation processes of ZIF-8.The materials were characterized by XRD and so on.Orthogonal experiment was used to determine the best loading technology for triptolide based on drug loading and encapsulation rate.Results ZIF-8 crystal prepared by the physical stirring method was excellent and stable,and the maximum specific surface area was 1622.38 m2·g-1.The best drug loading ratio was 5∶12 with drug loading concentration at 6 mg·mL-1,and loading for 3 days.Three batches of verification data showed that the drug loading and encapsulation efficiency of triptolide were(49.87±1.26)%and(70.66±1.26)%,respectively.Conclusion ZIF-8 can be successfully prepared by the physical stirring method with high drug loading and encapsulation efficiency for triptolide.The method is simple,and the process is stable.