微波辅助提取翘鳞香菇多糖及抗氧化性研究

Microwave-assisted extraction of Lentinus squarrosulus polysaccharides and its antioxidant ativity

研究以翘鳞香菇子实体多糖提取量为评价指标,采用微波辅助提取法,通过单因素试验和响应面试验优化翘鳞香菇多糖提取工艺,并研究翘鳞香菇多糖对DPPH·的清除作用。结果表明,由Box-Behnken试验优化后的提取工艺条件为微波时间155 s、微波功率600 W、液料比56 mL/g。经过验证试验,翘鳞香菇多糖提取量为24.13 mg/g(n=3,RSD=0.26%)。翘鳞香菇子实体多糖具有一定的DPPH·清除活性(IC50=0.70 g/L)。

Using polysaccharides extract amount from the fruiting body of Lentinus squarrosulus as the evaluation index.The study optimized microwave-assisted extraction of polysaccharides from the fruiting body of L.squarrosulus by single factor test and response surface methodology.Meanwhile,the scavenging effect of L.squarrosulus polysaccharides on DPPH radical was tested.The results indicated that the optimal extracting conditions were as follows:microwave time was 155 s,microwave power 600 W,liquid-solid ratio was 56 mL/g.Under the condition,the extraction amount of polysaccharides from the fruiting body of L.squarrosulus was 24.13 mg/g.The antioxidant tests results showed that scavenging capacity(IC50)of polysaccharides towards DPPH radical was 0.70 g/L,which indicated that polysaccharides had antioxidant activity.