摘要
研究以翘鳞香菇子实体多糖提取量为评价指标,采用微波辅助提取法,通过单因素试验和响应面试验优化翘鳞香菇多糖提取工艺,并研究翘鳞香菇多糖对DPPH·的清除作用。结果表明,由Box-Behnken试验优化后的提取工艺条件为微波时间155 s、微波功率600 W、液料比56 mL/g。经过验证试验,翘鳞香菇多糖提取量为24.13 mg/g(n=3,RSD=0.26%)。翘鳞香菇子实体多糖具有一定的DPPH·清除活性(IC50=0.70 g/L)。
Using polysaccharides extract amount from the fruiting body of Lentinus squarrosulus as the evaluation index.The study optimized microwave-assisted extraction of polysaccharides from the fruiting body of L.squarrosulus by single factor test and response surface methodology.Meanwhile,the scavenging effect of L.squarrosulus polysaccharides on DPPH radical was tested.The results indicated that the optimal extracting conditions were as follows:microwave time was 155 s,microwave power 600 W,liquid-solid ratio was 56 mL/g.Under the condition,the extraction amount of polysaccharides from the fruiting body of L.squarrosulus was 24.13 mg/g.The antioxidant tests results showed that scavenging capacity(IC50)of polysaccharides towards DPPH radical was 0.70 g/L,which indicated that polysaccharides had antioxidant activity.
作者
张鹏
花燕莹
梁樱子
唐森
谢济运
ZHANG Peng;HUA Yan-ying;LIANG Ying-zi;TANG Sen;XIE Ji-yun
出处
《饲料研究》
CAS
北大核心
2020年第12期74-79,共6页
Feed Research
基金
2018年度广西高校中青年教师基础能力提升项目(项目编号:2018KY0702)
2018~2020年广西本科高校食品科学与工程特色专业及实验实训教学基地(中心)建设项目(项目编号:桂教高教[2018]52号)
广西糖资源工程技术研究中心培育建设项目(项目编号:桂科AD16450040)
广西高校制糖工程综合技术重点实验室培育建设项目(项目编号:桂教科研[2016]6号)。
关键词
翘鳞香菇
子实体
多糖
响应面法
微波辅助
抗氧化
Lentinus squarrosulus
fruiting body
polysaccharides
response surface methodology
microwave-assisted
antioxidant