CT灌注成像评估兔后肢骨骼肌缺血模型缺血骨骼肌灌注状态

CT perfusion imaging in evaluation of perfusion status of rabbit hind limb skeletal muscle ischemia models

目的探讨CT灌注成像(CTPI)评估兔后肢骨骼肌缺血模型灌注状态的价值。方法选取48只新西兰大白兔,结扎并切除右侧股动脉及其分支建立后肢骨骼肌缺血模型,分别于术后第3、7、14、21、28、42天各随机取8只行健侧及术侧后肢CT扫描,以小腿肌肉最大横截面为中心层面进行灌注成像,获得组织时间-密度曲线(TDC),测量两侧骨骼肌血流量(BF)、血容量(BV)、平均通过时间(MTT)及达峰时间(PT)。结果健侧各时间点骨骼肌TDC均见曲线上升达峰值后迅速下降至相对平稳状态;术侧术后第28、42天曲线呈迅速上升后迅速下降至相对平稳水平,与术前曲线形态相近。术侧术后第3、7、14、21天BF、BV均低于健侧(P均<0.05);各时间点MTT、PT差异无统计学意义(P均>0.05)。结论CT灌注成像可用于评估兔后肢骨骼肌缺血模型灌注状态。

Objective To explore the value of CT perfusion imaging(CTPI)in evaluation of perfusion status in rabbit hind limb skeletal muscle ischemia models.Methods Totally 48 New Zealand white rabbits were selected.The right femoral artery and its branches were ligated and resected to establish the hind limb skeletal muscle ischemia model.Eight rabbits were randomly selected for CT scanning of the undamaged and operative hind limbs on the 3rd,7th,14th,21st,28th and 42nd postoperative days,respectively,and CTPI was performed taken the maximum cross section of calf muscle as the central layer.Time-density curve(TDC)and perfusion parameters such as blood flow(BF),blood volume(BV),mean transit time(MTT)and peak time(PT)of bilateral skeletal muscles were measured.Results On the healthy side,TDC curve of skeletal muscle increased to the peak and then decreased to a relatively stable state.On the 28th and 42nd postoperative days,the curve on the operative side increased rapidly and then decreased rapidly to a relatively stable level,which was similar to the curve before surgery.BF and BV on the 3rd,7th,14th,and 21st postoperative days on the operative side were lower than those on the healthy side(all P<0.05).There was no significant difference of MTT nor PT at all time points(all P>0.05).Conclusion CTPI could be used to monitor dynamic changes of skeletal muscles of rabbit hind limb skeletal muscle ischemia models.