孕妇尿中双酚A暴露水平与孕周的关联性分析

Associations between urinary bisphenol A concentrations during pregnancy and gestational age

【目的】研究孕妇孕期尿中双酚A(bisphenol A,BPA)浓度与孕周的关系。【方法】招募248名来自上海市某妇幼保健院的建卡的孕妇作为研究对象,完成问卷调查,并采集孕期尿液样本,采用气相色谱-串联质谱(GC-MS/MS)技术检测尿样中BPA的含量,并应用广义相加模型探究尿中BPA浓度与孕周的线性关系,以及多元线性回归模型探究尿中BPA浓度与孕周的关联性。【结果】孕妇尿样中BPA检出率为100%,浓度中位数和几何均数分别为0.85μg/L和1.21μg/L。孕期尿中BPA浓度与孕周关联呈线性(非线性检验P>0.05)。孕妇尿中BPA浓度与孕周呈显著性正相关(关联系数,β=0.19;95%CI:0.04~0.35;P=0.016);经性别分层后,仅在女性新生儿中观察到尿中BPA浓度与孕周的显著性正相关(β=0.18;95%CI:0.03~0.34;P=0.020)。孕程分层后,在孕中期和孕晚期采集尿液样本中均未观察到显著性关联。【结论】研究对象普遍暴露于BPA;孕期尿中BPA浓度可能增加分娩孕周,这一效应在女孩中更加明显。

[Objective]We aimed to assess the association between urinary bisphenol A(BPA)concentrations and gestational age in pregnant women.[Methods]A total of 248 pregnant women were recruited from a maternal and child care hospital in Shanghai.A questionnaire survey was completed to collect socio-demographic information and spot urine samples were collected during pregnancy.Gas chromatography-tandem mass spectrometry(GC-MS/MS)was used to measure BPA concentrations in urine samples.Linear relationship between urinary BPA level and gestational age was assessed by using generalized additive models.Multivariate regression model was used to evaluate associations of prenatal BPA exposure with gestational age.[Results]BPA was detected in all the urine samples.Median value and geometric mean of urinary BPA levels were 0.85μg/L and 1.21μg/L,respectively.Linear relationship between urinary BPA concentration and gestational weeks was confirmed(non-linear P>0.05).Positive association between urinary BPA level and gestational age was indicated(regression coefficient,β=0.19;95%CI:0.04-0.35;P=0.016).However,it was only observed in girls,stratified by sex of newborns(β=0.18;95%CI:0.03-0.34;P=0.020).After stratification by trimester,no significant association was found in the second or the third trimesters.[Conclusion]Pregnant women are extensively exposed to BPA.Urinary BPA exposure during pregnancy may extend gestational age,especially in girls.