IPN 智能水凝胶药物缓释体系的设计及评价

Design and Evaluation of IPN Intelligent Hydrogels for Sustained and Controlled Drug Delivery System

为研究水凝胶的缓释作用,制备了一种新型pH敏感型互穿网络(IPN)水凝胶药物缓释体系。以天然多糖材料海藻酸钠(SA)和壳聚糖(CS)为原料,利用高碘酸钠氧化SA引入醛基与CS的水溶性衍生物羧化壳聚糖(CMCS)进行化学交联,同时将SA和钙离子进行物理交联,外层再用CS复合凝聚形成聚电解质膜覆盖,从而得到以多种方式交联的具有IPN结构的缓释水凝胶。采用多种方法表征水凝胶的物理化学结构,并探索了复合水凝胶的最佳性能条件,通过溶胀实验和释药测试研究其性能。结果表明,当氧化海藻酸钠(OSA)和CMCS的共混比例为2∶3、氯化钙溶液浓度为2%、CS浓度为2%、包膜时间为30min时,水凝胶的最大溶胀率可以达到33.59。此水凝胶对模型药物牛血清白蛋白(BSA)、吲哚美辛(IDM)有明显缓释作用,说明此复合水凝胶有望开发成一种集多种交联方式为一体的新型药物缓控释系统。

In order to study the sustained release effect of hydrogels,a new pH-sensitive sustained drug release system based on hydrogels with interpenetrating network(IPN)was prepared.The polysaccharides of sodium alginate(SA)and chitosan(CS)were used as raw materials.SA was oxidated by sodium periodate to introduce the aldehyde group to crosslink chemically with the soluble CS derivative of carboxylation chitosan(CMCS).Meanwhile,SA was physically crosslinked by calcium ions.The surfaces of the hydrogels were coated with the polyelectrolyte membrane by interaction with CS.Hence,multiple crosslinking methods were introduced to form the IPN sustained release hydrogels.The physicochemical properties of the hydrogel were characterized by FTIR,TGA,XRD,and SEM.And the optimal performance conditions of the hydrogels were explored.The result indicates that the blend ratio of oxidation of sodium alginate(OSA)and CMCS is 2:3,both the concentration of CaCl2 and CS are 2%,the coating time keeps 30 min,then the swelling properties of the hydrogels was optimal with the swelling rate of 33.59.This hydrogels exhibit good sustained-release effect on bovine serum albumin(BSA)and indomethacin(IDM),and are expected to develop a new sustained and controlled drug release system.