水杨酸分子印迹聚合物整体柱合成及其固相微萃取应用

Synthesis and application of salicylic acid imprinted polymer monolithic column for solid phase microextraction

以水杨酸为模板分子,4-乙烯吡啶为功能单体,在模板分子∶功能单体∶交联剂为1∶4∶18的条件下,运用原位聚合法在移液枪头中合成了水杨酸印迹聚合物整体柱,并应用于水杨酸的固相微萃取.优化了上样溶液体积、pH、洗脱剂种类、体积、萃取流速等萃取条件,建立了固相微萃取-高效液相色谱定量分析方法.实验结果显示,检出限(LOD)为1.2 ng/mL,回收率的范围92.1%~105.8%,日内、日间精密度(RSD)均小于9.8%.该方法简单快速、选择性好、灵敏度高,适合于环境水样中水杨酸的分离分析.

Under condition of the template molecule∶functional monomer∶crosslinking agent for 1∶4∶18,saliva acid as a template molecule and 4-ethylene pyridine as a functional monomer were used to synthesis of saliva acid imprinting polymer column and applied to the solid phase micro-extraction of saliva.The extraction conditions such as supersolution liquid product,pH,type of wasting agent,volume and extraction flow rate were optimized,and the quantitative analysis method of solid-phase micro-extraction-high performance liquid chromatography was established.The experimental results showed that the detection limit(LOD)was 1.2 ng/mL,the recovery rate ranged from 92.1%to 105.8%,and the inter-day and intra-day precision(RSD)were less than 9.8%.The method is simple and fast,high selective and high sensitivity,and suitable for the separation analysis of salient acid in ambient water samples.