小球藻与硝化细菌对食品厂污水中氨氮和总氮的去除研究

Studies on removal of ammonia nitrogen and total nitrogen from food factory sewage by chlorella and nitrifying bacteria

利用BG11培养基和硝化细菌培养基分离到小球藻和两株硝化细菌XHL1和XHL12,采用扫描电镜对小球藻外部形态进行观察,通过计数方法制作小球藻生长曲线,对分离到的两株硝化细菌绘制细菌系统发育树。结果表明:分离到的小球藻直径为4.5μm,系统发育树结果显示XHL1和XHL12均属于Acinetobacter sp baumannii.属,分别与Acinetobacter strain SO20(HQ631961)和Acinetobacter sp.2C56(JN228308)的同源性最高,分别为100%和99%。单独培养时,小球藻对污水中氨氮的去除率为94.18%,总氮的去除率为84%。小球藻-XHL1共处条件下氨氮的去除效率为99.3%,总氮的去除率为78%。分离到的小球藻和硝化细菌对污水中氨氮和总氮具有较高的去除能力。

Chlorella and two strains of nitrifying bacteria XHL1 and XHL12 were isolated by using BG11 and nitrifying bacteria mediums.The external morphology of chlorella was observed by scanning electron microscope(SEM)and the growth curve of chlorella was obtained by counting algae cells.The phylogenetic tree of XHL1 and XHL12 was mapped.The results show that the diameter of chlorella is 4.5 microns.Phylogenetic tree results show that the two strains XHL1 and XHL12 belong to the genus of Acinetobacter sp baumannii,and have the highest homology with Acinetobacter strain SO20(HQ631961)and Acinetobacter sp.2C56(JN228308)respectively,being 100%and 99%.When they are cultivated separately,the chlorella’s removal rates of ammonia nitrogen and total nitrogen in wastewater are 94.18%and 84%respectively.Under the conditions of chlococcus-XHL1,the ammonia nitrogen removal rate is 99.3%and the total nitrogen removal rate was 78%.Chlorella and nitrifying bacteria isolated in this study have high removal capacity for ammonia and total nitrogen in sewage.