玉米DUF581基因家族的分子进化及干旱胁迫下表达模式

The molecular evolution of maize DUF581 gene family and expression patterns in response to drought stress

利用生物信息学方法,从玉米中鉴定到45个DUF 581基因家族成员,与其他物种该家族基因的比较和系统发育分析表明,DUF 581基因在物种间理化性质较保守;基于系统发育树,该家族基因可分为Ⅰ~Ⅵ6个进化枝,进化枝Ⅱ~Ⅵ内的玉米DUF 581基因结构保守,含有1~2个外显子,而进化枝Ⅰ内的家族成员平均含有5个外显子;玉米DUF 581基因散布于10条染色体上;基因复制分析表明玉米的14个串联复制基因位于4个串联复制基因簇中,两对片段复制基因Zm00001d002530/Zm00001d017646和Zm00001d017646/Zm00001d051496同时又属于串联复制基因;在正常生长条件下,接近半数(22/45)的玉米DUF 581基因在玉米的3种组织(叶、雌穗和雄穗)、4个生长发育时期(V12、V14、V18和R1期)内呈现组成型表达,并且在雌穗和雄穗两个生殖组织内有较高的表达水平;干旱胁迫下,26个DUF 581呈现差异表达,V14和V18期的雌穗以及V12和V14期的雄穗中差异表达基因都被下调,而雌穗V12和R1期的大部分差异表达基因都被上调;此外,大部分复制基因对表现出差异的表达模式。

The 45 members of DUF581 gene family in maize were identified using bioinformatics methods in this study,and the sequence analysis of the DUF581 genes showed the physiochemical properties of the gene family were conserved among different species.Based on the phylogenetic tree,the family genes were divided into six clades,CladeⅠ~Ⅵ.The structure of the maize DUF581 gene in clades Ⅱ~Ⅵwas similar and conserved,and these members contained 1 to 2 exons;while the number of exons of family members in clade I contained five on average.Maize DUF581 genes were distributed on 10 chromosomes.Gene duplication events analysis showed that 14 tandem duplication genes in maize were located in 4 tandem duplication gene groups.Two segmental duplication gene pairs Zm00001d002530/Zm00001d017646 and Zm00001d017646/Zm00001d051496 were also tandem duplications.Expression pattern analysis of maize DUF581 genes in three tissues(leaf,ear,and tassel)at four developmental stages(V12,V14,V18,and R1)under normal growth conditions demonstrated that nearly half(22/45)of the maize DUF581 gene were ubiquitously expressed in three tissues at four stages,and had higher expression levels in the two reproductive tissues of ear and tassel.A total of 26 differentially expressed genes(DEGs)were identified under drought stress.All the DEGs in ear at V14 and V18 stages,and tassel at V12 and V14 stages were down-regulated,while most of the DEGs in ear at V12 and R1 stages were up-regulated,and more,most of the duplication pairs showed different expression patterns.