高锰酸钾胁迫及毒后恢复对锦鲤肝脏组织的影响

Effects of potassium permanganate stress and post-toxic recovery on liver tissue of Cyprinus Carpio

为了解高锰酸钾消毒对锦鲤幼鱼毒性毒理的影响,采用静水生物测试法研究高锰酸钾(KMnO 4)对锦鲤幼鱼的急性毒性并进行安全评价。以(16.36±0.01)g锦鲤幼鱼为试验对象,设定0.5、1、2 mg·L^-1和3 mg·L^-14个KMnO 4浓度梯度进行48 h急性暴露试验,统计累计死亡率,将剩余幼鱼进行48 h恢复试验。在胁迫0、24 h、48 h和恢复24 h、48 h等5个时间段取样,以肝脏组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷草转氨酶(GOT)活性以及谷胱甘肽(GSH)和丙二醛(MDA)含量为指标,研究KMnO 4对锦鲤幼鱼的毒理作用。结果表明:2 mg·L^-1以上的高锰酸钾溶液浸泡24 h能使锦鲤幼鱼致死,SOD、CAT、GOT受到抑制。在解除胁迫后基本能恢复到正常水平。而GHS和MDA含量在胁迫过程中是呈升高趋势的,但在解除胁迫后均下降到与对照组相似水平。研究结果表明KMnO 4会造成锦鲤肝脏的损伤,且损伤程度随着浓度和时间的增加而加重,但是解除胁迫后,SOD、CAT、GOT和GSH、MDA等均能达到不同程度的恢复。使用溶液浓度为1 mg·L^-1的高锰酸钾对锦鲤幼鱼消毒最为合适。

In order to understand the toxicological effect of potassium permanganate disinfection on juvenile koi,the acute toxicity of potassium permanganate(KMnO 4)to juvenile koi was studied by static water bioassay and safety evaluation was carried out.The acute exposure experiment of 48 h was carried out with 0.5,1,2 mg·L^-1 and 3 mg·L^-1 KMnO 4 concentration gradients,and the cumulative mortality was calculated,and the 48 h recovery experiment for the rest of fist was carried out.The activities of superoxide dismutase(SOD),catalase(CAT)and glutamic oxaloacetic transaminase(GOT)and the contents of glutathione(GSH)and malondialdehyde(MDA)in liver tissue were taken at 0,24 and 48 hours after stress and 24 hours and 48 hours after recovery.The toxicological effects of KMnO 4 on juvenile koi were studied.The results showed that soaking in 2 mg·L^-1 potassium permanganate solution for 24 hours could kill juvenile koi and inhibit SOD,CAT and GOT.After relieving the stress,it can basically return to the normal level.The contents of GHS and MDA increased in the process of stress,but decreased to a level similar to that of the control group after relieving stress.The results showed that KMnO 4 could cause liver damage of koi,and the degree of damage was aggravated with the increase of concentration and time,but after relieving stress,SOD,CAT,GOT,GSH,MDA and so on could recover to different degrees.Potassium permanganate with the concentration of 1 mg·L^-1 is the most suitable for disinfection of juvenile koi.