哮喘伴肺炎支原体感染患儿RBP、IgE、IFN-γ、25(OH)D表达水平及其临床意义

Expression levels of RBP,IgE,IFN-γand 25(OH)D and their clinical significance in children with infantile asthma complicated with mycoplasma pneumoniae infection

目的探究哮喘伴肺炎支原体(Mycoplasma pneumoniae,MP)感染患儿视黄醇结合蛋白(Retinol binding protein,RBP)、免疫球蛋白E(Immunoglobulin E,IgE)、γ-干扰素(γ-interferon,IFN-γ)、25羟维生素D[25 hydroxyvitamin D,25(OH)D]表达水平及其临床意义。方法选取2017年12月-2019年12月间于承德医学院附属医院、承德钢铁集团有限公司职工医院小儿内科接受治疗的140例哮喘伴肺炎支原体感染患儿作为试验组,另选取同期健康体检儿童60名作为对照组。检测并比较两组研究对象血清细胞因子水平,并比较不同MP抗体(MP-IgM)滴度和不同哮喘分期上述指标水平,分析上述指标与MP-IgM滴度的关系。结果试验组血清RBP、25(OH)D水平均低于对照组,血清IgE和IFN-γ水平均高于对照组(P<0.05)。MP-IgM高滴度组血清RBP和25(OH)D水平均低于低滴度组,血清IgE和IFN-γ水平均高于低滴度组(P<0.05)。急性发作期组血清RBP、25(OH)D水平均低于病情缓解期组,血清IgE、IFN-γ水平均高于病情缓解期组(P<0.05)。MP-IgM滴度与RBP和25(OH)D呈负相关关系(r=-0.416、-0.437,P<0.05),与IgE和IFN-γ呈正相关关系(r=0.592、0.501,P<0.05)。结论哮喘伴MP感染患儿血清RBP、25(OH)D水平降低,血清IgE和IFN-γ水平升高,且血清RBP、IgE、IFN-γ、25(OH)D可作为临床诊断哮喘伴MP感染的客观指标。

OBJECTIVE To study the expression levels and clinical significance of retinol binding protein(RBP),immunoglobulin E(IgE),interferon-γ(IFN-γ)and 25-hydroxyvitamin D[25(OH)D]in children with asthma complicated with mycoplasma pneumoniae(MP)infection.METHODS Total of 140 children with infantile asthma complicated with MP infection who were treated in the pediatric internal medicine department of the Affiliated Hospital of Chengde Medical College and Staff Hospital of Chengde Iron and Steel Group Co,Ltd between Dec.2017 and Dec.2019 were recruited as the observation group,and 60 healthy children during the period were enrolled as the control group.The levels of serum factors between the two groups were detected and compared;the index levels in patients with different MP-IgM titers and asthma stages were compared,and the correlation between them was analyzed.RESULTS The levels of serum RBP and 25(OH)D in the observation group were significantly lower than that in the control group,whereas the levels of serum IgE and IFN-γin the observation group were significantly higher than that in the control group(P<0.05).The levels of serum RBP and 25(OH)D in patients with high MP-IgM titer were significantly lower than that with low MP-IgM titer,and the levels of serum IgE and IFN-γwere higher than that with MP-IgM low titer(P<0.05).The levels of serum RBP and 25(OH)D in patients in acute attack period were significantly lower than that in remission period,whereas the levels of serum IgE and IFN-γwere significantly higher than that in remission period(P<0.05).MP-IgM titer was negatively correlated with RBP and 25(OH)D(r=-0.416,-0.437,P<0.05),and positively correlated with IgE and IFN-γ(r=0.592,0.501,P<0.05).CONCLUSION The levels of serum RBP and 25(OH)D in children with infantile asthma complicated with MP infection decreased,whereas the levels of serum IgE and IFN-γincreased.The expression levels of serum RBP,IgE,IFN-γand 25(OH)D can be used as the objective indicators for clinical diagnosis of infantile asthma with MP infection.