不同染色法对甲状腺细针穿刺细胞学检查的影响

Effect of different staining methods on thyroid fine needle aspiration cytology

目的比较四种染色法在甲状腺细针穿刺细胞学涂片的应用效果。方法回顾145例甲状腺乳头状癌病例术前用四种染色制作的细针穿刺细胞涂片,比较四种染色法的技术特点、染色效果及诊断结果。结果四种方法染色效果均能满足诊断需要,诊断阳性符合率均高于90%,误诊率为0;与苏木素单染色和HE染色相比,BASO和DIFF染色制片耗时明显减少;而苏木素染色涂片背景最为干净,细胞核形态最为突出醒目,观察更省力。结论BASO和DIFF染色适合作为快速甲状腺细针穿刺细胞学检查的制片方法,苏木素单染法更适合大批量常规甲状腺细针穿刺细胞学检查。

Objective To compare the effect of thyroid fine needle aspiration cytology(FNAC)smears by 4 kinds of staining methods.Methods A total of 145 thyroid FNAC cases by 4 kinds of staining methods with histological results of PTC were reviewed.Technical characteristics,dyeing effect and diagnosis results were analyzed.Results All of 4 staining methods were able to meet the needs of diagnosis,the diagnostic positive coincidence rates were all higher than 90%,and misdiagnosis rates were 0.Compared to conventional HE staining and single hematoxylin staining,the production times of BASO and DIFF staining were significantly reduced;the background of smears by single hematoxylin staining was the cleanest and the nuclear characteristics were more clear and prominent.Conlusion BASO and DIFF stains are suitable for rapid thyroid FNAC,and hematoxylin single staining is more suitable for massive routine FNAC.