基于HPLC指纹图谱结合灰色关联度与聚类分析对不同产地石榴皮质量评价研究

Quality Evaluation of Granati Pericarpium from Different Areas with Grey Correlation and Cluster Analysis based on HPLC Fingerprint

目的对采自不同产地的11批次石榴皮样品建立HPLC指纹图谱,确定了21个色谱峰为共有峰,测定了石榴皮中主要成分的含量,利用灰色关联度结合聚类分析与判别分析,建立一种新型石榴皮质量综合评价方法。方法采用甲醇-0.1%磷酸为流动相,梯度洗脱,建立11批次石榴皮的高效液相指纹图谱;采用HPLC测定石榴皮中没食子酸、安石榴苷和鞣花酸的含量;利用灰色关联结合聚类分析与判别分析相结合的方法对指纹图谱和化学成分进行量化数据处理。结果建立的11批次石榴皮HPLC指纹图谱相似度良好,灰色关联分析结果表明S5(江苏)、S1(四川)、S3(河北)和S8(安徽)的药用价值更好;通过聚类分析发现11批次石榴皮的药用价值可分为5类,其中S5(江苏)为第一类,S7(陕西)和S8(安徽)为第二类,S1(四川)、S2(江苏)、S3(河北)分为第三类,两种分析结果相互印证、相互补充,同时判别分析进一步验证了聚类分析的结果,分析结果科学严谨。结论HPLC指纹图谱结合灰色关联度与聚类分析与判别分析对11批次不同产地的石榴皮进行质量评价,所建立的方法准确可靠,可用于石榴皮药材内在质量的综合评价,为石榴皮适宜产地的优选及其质量控制方法的规范化和标准化提供基础资料。

OBJECTIVE To establish a new comprehensive method to evaluate the quality of Granati Pericarpium collected from 11 different regions by HPLC fingerprint combined with grey correlation analysis,cluster analysis and discriminant analysis.METHODS HPLC fingerprint was established with mobile phase consisting of methanol and 0.1%phosphoric acid,and the contents of gallic acid,punicalagin and ellagic acid were determined,while the results of the HPLC fingerprint and chemical composition of Granati Pericarpium were processed by grey correlation,cluster and discriminant analysis.RESULTS The HPLC fingerprint of the samples had good similarity.Twenty-one common peaks were confirmed.The grey correlation analysis showed that S5(Jiangsu),S1(Sichuan),S3(Hebei)and S8(Anhui)had better medical value.The cluster analysis indicated that the Granati Pericarpium collected from 11 different regions could be divided into five categories,among which S5(Jiangsu)belonged to the first class S1(Sichuan),S2(Jiangsu)and S3(Hebei)belonged to the third class,S7(Shanxi)and S8(Anhui)belonged to the second class.The results of three kinds of analysis were mutually validated and complementary.CONCLUSION HPLC fingerprint combined with gray correlation,cluster and discriminant analysis can comprehensively evaluate the quality of Granati Pericarpium.This paper establishes a scientific,accurate and reliable method,which can be used for comprehensive evaluation of the internal quality of Granati Pericarpium.