BD Kiestra InoqulA全自动标本处理系统在临床微生物检验中的应用研究

Study on the application of BD Kiestra InoqulA fully automatic sample processing system in clinical microbiology laboratory

目的评价BD Kiestra InoqulA全自动标本处理系统接种临床尿液和血液/无菌体液标本的效果。方法分别用BD Kiestra InoqulA(仪器法)和手工法同时接种尿液标本810例和阳性血/无菌体液培养标本120例,35℃培养24 h观察菌落生长情况,比较两种接种方法培养阳性率、分离得到的单个菌落数、菌落计数及菌种数的差异。结果930例标本中,仪器法接种的培养阳性率为37%,手工法为38%,两者比较差异无统计学意义(P>0.05);两种接种方法均为阳性的339例标本中,仪器接种比传统手工接种得到更多的单个菌落,差异有统计学意义(P<0.05);从菌落总数和菌种数来看,仪器法接种的尿培养标本菌落总数大于或等于102 cfu/mL,分离到2种菌的标本都要比手工法多;而分离到阳性血和/无菌体液菌量大于或等于105 cfu/mL的标本例数仪器法比手工法更多。结论BD Kiestra InoqulA全自动微生物标本处理系统是微生物实验室高效的自动化仪器。

Objective To evaluate the efficacy of BD Kiestra InoqulA automated sample processing system for inoculating clinical urine and blood/sterile body fluid specimens.Methods BD Kiestra InoqulA(instrumental method)and manual method were used to simultaneously inoculate 810 urine samples and 120 positive blood/sterile body fluid samples.The samples were cultured at 35℃for 24 h to observe the colony growth.The positive rate of the two inoculation methods,the number of isolated colonies,the count of colonies and the number of species were compared.Results Among the 930 samples,the positive rate of instrumental inoculation was 37%and that of manual inoculation was 38%,showing no statistical difference(P>0.05).Among the 339 samples with positive results of both inoculation methods,more individual colonies were isolated by instrumental inoculation than by traditional manual inoculation,and the difference was statistically significant(P<0.05).From the perspective of the total number of colonies and the number of strains,the total number of colonies≥102 cfu/mL in the urine samples inoculated by the instrument method was larger than that by the manual method.And more samples of the two strains were isolated from the urine samples inoculated by the instrument method than by the manual method.The number of samples with 105 cfu/mL of positive blood/body fluid isolated by instrumental method was more than manual method.Conclusion BD Kiestra InoqulA′s fully automatic sample processing system is an efficient automatic instrument in microbiology laboratory.