基于ITS和ycf1序列的白及遗传多样性分析

Genetic Diversity Analysis of Bletilla striata Based on ITS and ycf1 Genes

在白及资源调查和收集过程中,发现白及花型、花色、花期、叶片形态等多个性状变异程度较大。本研究收集了来源于四川和云南的白及资源30份,克隆并分析其nrDNA ITS和叶绿体ycf1基因差异,发现ycf1基因差异小,ITS基因差异较大,根据ITS序列差异,将白及聚类为3个支系,结合白及的表观性状,发现白及ITS序列与其花期和叶型有相关性,与花型、花色等其他性状无明显相关,本研究为白及种质资源收集整理和开发利用提供了理论基础。

During the investigation and collection of Bletilla striata resources,it was found that many characteristics of Bletilla striata such as flower type,flower color,florescence,leaf shape and so on had great variation degrees.In this study,we collected 30 accessions of Bletilla striata resources from Sichuan and Yunnan province,cloned and analyzed their nrDNA ITS and chloroplast ycf1 gene differences,and found that ycf1 gene has little difference and ITS gene has large difference.According to the differences of ITS sequences,Bletilla striata was clustered into 3 branches.Combined with the apparent characteristics of Bletilla striata,it was found that the ITS sequences of Bletilla striata had obvious correlation with florescence and leaf type,but had no obvious correlation with other characteristics such as flower type and color.This study provided a theoretical basis for the collection,development and utilization of Bletilla striata germplasm resources.