实验材料检测在SPF级实验小鼠质量控制中的应用

Application of Experimental Material Inspection in Quality Control of SPF Mice

目的探讨对SPF级实验小鼠屏障设施进行实验材料检测的可行性及应用价值。方法收集带入屏障设施的实验材料,对非细胞样品进行细菌培养,对细胞样品用PCR法检测支原体。结果5年共收集14708例实验材料,其中非细胞样品13085例,细菌平均检出率为1.99%;细胞样品1623例,支原体平均检出率为4.81%。整体上阳性检出率呈逐年下降趋势,近年来稳定在一个相对较低的水平。结论对带入屏障设施的实验材料进行大规模持续检控切实可行,可提示研究人员加强对动物实验材料的无菌处理,可使管理者确定来自实验材料的危害程度,同时也是SPF级实验小鼠微生物感染防控的有效措施之一。

Objective To establish inspection methods for materials carried into barrier facilities housing specific pathogen-free(SPF)laboratory mice.Methods Experimental materials brought into a murine barrier facility were collected.Non-cellular material samples were cultured to identify whether samples harbored bacteria,and cell samples were screened for the presence or absence of mycoplasma using PCR.Results A total of 14708 experimental materials were collected and evaluated over a 5-year period.The average positive rate for bacterial detection in 13085 non-cellular samples was 1.99%,and the positive rate for mycoplasma in 1623 cell samples was 4.81%.Positive detection rates for bacteria and mycoplasma have gradually decreased and have remained at low levels over recent years.Conclusion sIt is feasible to conduct continuous large-scale inspection,monitoring,and control of experimental materials used in animal barrier facilities.Inspection processes can strengthen the confidence of researchers performing aseptic treatment with animal experimental materials and enable facility managers to evaluate risks from experimental materials.Experimental material inspection is an effective measure for microbial quality control in SPF experimental mice.