miR-155通过调控CaSR表达影响VSMC生长的机制研究

Study on the mechanism of miR-155 regulating CaSR expression and affecting the growth of vascular smooth muscle cells

目的miR-155可以通过干扰血管平滑肌细胞(VMSC)生物活性来抑制动脉粥样硬化斑块的形成,但具体机制不十分清楚。本研究主要观察miR-155对VSMC中钙敏感受体(CaSR)表达的影响,同时探讨CaSR在miR-155调控VSMC生物活性过程中的作用。方法培养VSMC,将miR-155 mimic、Ad-CaSR转染到VSMC中,Western blot检测相关基因的表达,MTT测定VSMC生长情况,流式细胞术检测VSMC的凋亡情况,Transwell检测VSMC迁移情况。结果miR-155 mimic组CaSR的表达水平明显受抑制,VSMC凋亡明显减少,而VSMC的增殖和迁移能力明显增强;Ad-CaSR组和miR-155+Ad-CaSR组VSMC凋亡明显增加,而VSMC的增殖和迁移能力明显下降。结论miR-155可以调控VSMC的生长,该作用可能是通过影响VSMC中CaSR的活性得以实现。

Aim miR-155 can inhibit the formation of atherosclerotic plaques by interfering with the biological activity of vascular smooth muscle cells,but the specific mechanism is not very clear.The purpose of this study was to observe the effects of miR-155 on calcium sensing receptor(CaSR)expression in vascular smooth muscle cells,and to explore the role of CaSR in miR-155 in regulating the biological activity of vascular smooth muscle cells.Methods Vascular smooth muscle cells were cultured,and miR-155 mimic and Ad-CaSR were transfected into vascular smooth muscle cells.Western blot was used to detect the expression of related genes.MTT was used to determine the growth of vascular smooth muscle cells.Flow cytometry was used to detect the apoptosis of vascular smooth muscle cells.Transwell was used to detect the migration of vascular smooth muscle cell.Results The expression level of CaSR in miR-155 mimic group was significantly inhibited,the apoptosis of vascular smooth muscle cells was significantly reduced,and the proliferation and migration capabilities of vascular smooth muscle cells were significantly enhanced.Apoptosis of the cells was significantly increased,while the proliferation and migration capacity of vascular smooth muscle cells was significantly reduced.Conclusion miR-155 can regulate the growth of vascular smooth muscle cells,but this effect may be achieved by affecting the activity of CaSR in vascular smooth muscle cells.