基于网络药理学探讨连花清瘟胶囊防治新型冠状病毒肺炎(COVID-19)的作用机制

TO EXPLORE THE MECHANISM OF LIANHUA QINGWEN CAPSULE IN THE PREVENTION AND TREATMENT OF COVID-19 BASED ON NETWORK PHARMACOLOGY

目的基于网络药理学方法研究连花清瘟胶囊活性成分防治新型冠状病毒肺炎的作用机制。方法通过文献挖掘确定连花清瘟胶囊活性成分,依托TCMSP、Swiss Target Prediction数据库检索活性成分靶点,利用Cytoscape3.6.1软件绘制连花清瘟胶囊活性成分-靶点网络;运用GeneCards数据库查询新型冠状病毒肺炎靶点,将其与连花清瘟胶囊活性成分靶点相互映射,筛选出共同靶点。应用STRING平台构建靶蛋白相互作用网络并提取关键靶点,对关键靶点进行GO(Gene Ontology)基因功能和通路分析。结果连花清瘟胶囊24个活性成分作用于33个新型冠状病毒肺炎靶点,其中NOS2与血管紧张素转化酶II(ACE2)共表达;TNF、TP53、IL6、CASP3、IL2、MAPK14、RELA共7个关键靶基因参与免疫系统过程、多细胞生物过程、解毒过程、刺激反应、抗氧化活性等生物过程及功能,调控百日咳、南美锥虫病、乙型肝炎、肺结核、甲型流感、EB病毒感染、单纯疱疹病毒感染、PI3K-Akt、TNF、MAPK、T细胞受体等信号通路。结论连花清瘟胶囊对SARS-CoV-2入侵人体受体ACE2具有一定的阻断作用,同时发挥抑制机体炎症风暴、调节机体免疫的作用。

Objective:To investigate the mechanism of active constituents of Lianhua Qingwen capsule in the prevention and treatment of Novel Coronavious Pneumonica based on network pharmacology.Methods:Active constituents of Lianhua Qingwen capsule were determined by literature mining,and targets of active constituents were retrieved based on TCMSP and Swiss Target Prediction database.Cytoscape3.6.1 software was used to draw the network of active constituents-targets.GeneCards database was used to query targets of Novel Coronavious Pneumonica,and targets of its active components of Lianhua Qingwen capsule were mapped to each other to screen out common targets.Using STRING platform to construct target genes interaction network and extract key targets,so as to analyze the GO(Gene Ontology)gene function and pathway of key targets.Results:24 active constituents of Lianhua Qingwen capsule acted on 33 targets of Novel Coronavious Pneumonica,among which NOS2 was co-expressed with angiotensin-converting enzyme II.TNF,TP53,IL6,CASP3 IL2,MAPK14 and RELA,a total of seven key target genes involved in the immune system process,multicellular organisms,detoxification process,stimulus-response,antioxidant activity and other biological processes and functions;regulating Pertussis,Chagas disease,Hepatitis B,Tuberculosi,Influenza A,Epstein-Barr virus infection,Herpes simplex infection n,PI3K-Akt signaling pathway,TNF signaling pathway,MAPK signaling pathway,T cell receptor signaling pathway and other signaling pathways.Conclusions:Lianhua Qingwen capsule had a certain blocking effect on the invasion of human receptor ACE2 by SARS-CoV-2,at the same time,which played a role in inhibiting inflammatory storm and regulating the body's immunity.