“关-开”型荧光探针测定人体尿液中丙酮

Determination of acetone in human urine based on“off-on”fluorescent probes

碘(I3-)与罗丹明B(RhB)缔合作用使罗丹明B荧光信号强度减弱,丙酮碘仿反应使体系荧光信号再现,再现程度与丙酮浓度成线性关系。据此建立了以RhB-I-3缔合物为荧光探针测定尿液中丙酮浓度的方法,在激发波长365 nm、发射波长580 nm条件下进行了丙酮的荧光测定。结果表明,在0~5μmol/L和5~12μmol/L范围内存在线性方程:△F=-0.0428+3.77c(μmol/L);△F=-86.42+19.68c(μmol/L),检出限为7.96 nmol/L。丙酮与酸性KMnO4溶液不反应,酸性KMnO4处理尿液将有效克服共存还原性物质的干扰,方法用于尿液丙酮含量分析,加标回收率在94.8%~97.5%之间,正常人和糖尿病患者尿液丙酮含量有明显差异。

The association of rhodamine B(RhB)with iodine(I3-)is capable to degrade the fluorescence signal intensity,while the acetone iodoform reaction reproduces the fluorescence signal of RhB,and the reproduction degree is linearly correlating to the acetone concentration.Herein,a new method using Rh B-I3-complex as fluorescent probe to detect the acetone concentration in urine was developed,in which the excitation and emission wavelengths were 365 nm and 580 nm,respectively.The results illustrated the linear equations of△F=-0.0428+3.77 c(μmol/L)and△F=-86.42+19.68 c(μmol/L)in the concentration ranges of0-5μmol/L and 5-12μmol/L,respectively,and the detection limit was 7.96 nmol/L.Owing to the unresponsiveness between acetone and acidic potassium permanganate,the interference to co-existed reduced substances was effectively conquered.This work provided a simple and fast acetone analysis in urine,and the recoveries were between 94.8%and 97.5%,showing a potential of detecting the acetone content in urine between normal people and diabetic patients.