一种针对新型冠状病毒肺炎的准确、快速、便携式双功能电检测仪

An accurate,high-speed,portable bifunctional electrical detector for COVID-19

新型冠状病毒肺炎(COVID-19)正在多个国家快速传播,已经导致了严重的全球大流行.由于目前没有针对此类病人的特效药和针对此病毒的疫苗,准确、快速地进行新冠病人检测成为了控制大流行最有效的措施.本文中我们开发了一种基于石墨烯场效应晶体管的便携式双功能电检测仪,其通过核酸互补杂交或者抗原-抗体特异性结合作用,能分别进行病毒核酸序列检测和抗体检测,检测限分别低至0.1和1 fg mL^(-1).我们通过临床样品检测进一步评估了此方法:从10个新冠病人和8个正常人咽拭子中提取RNA直接用于核酸检测;从6个新冠病人和3个正常人血清中提取抗体用于抗体检测.临床样品检测结果和基于聚合酶链反应的光学方法结果完全吻合,同时此方法拥有超快的检测速度(核酸检测需10 min,抗体检测需5 min).因此,我们的实验提供了一种有效、准确、高通量的新冠现场即时检测工具.

Coronavirus disease 2019(COVID-19),caused by SARS-CoV-2,has rapidly spread and caused a severe global pandemic.Because no specific drugs are available for COVID-19 and few vaccines are available for SARS-CoV-2,accurate and rapid diagnosis of COVID-19 has been the most crucial measure to control this pandemic.Here,we developed a portable bifunctional electrical detector based on graphene fieldeffect transistors for SARS-CoV-2 through either nucleic acid hybridization or antigen-antibody protein interaction,with ultra-low limits of detection of~0.1 and~1 fg mL^(−1) in phosphate buffer saline,respectively.We validated our method by assessment of RNA extracts from the oropharyngeal swabs of ten COVID-19 patients and eight healthy subjects,and the IgM/IgG antibodies from serum specimens of six COVID-19 patients and three healthy subjects.Here we show that the diagnostic results are in excellent agreement with the findings of polymerase chain reaction-based optical methods;they also exhibit rapid detection speed(~10 min for nucleic acid detection and~5 min for immunoassay).Therefore,our assay provides an efficient,accurate tool for high-throughput point-of-care testing.