摘要
长链非编码RNA(lncRNA)在免疫及病毒与宿主互作中发挥重要作用。本研究在猪PAM细胞中发现一种新的猪长链非编码RNA lnc-000649,其全序列长1 483 bp。利用荧光定量PCR技术检测了猪繁殖与呼吸综合征病毒(PRRSV)感染前后PAM细胞中lnc-000649的表达水平,发现其在PRRSV感染后表达显著(P<0.01)下调。为了进一步研究该lncRNA的功能,构建了猪lnc-000649超表达载体lnc-000649-pcDNA3.1,并转染PAM细胞,成功构建了lnc-000649超表达细胞模型。经PRRSV接种后发现,转染lnc-000649-pcDNA3.1组的PAM细胞内PRRSV RNA含量极显著(P<0.01)低于转染pcDNA3.1(+)空载体和未转染组,说明猪lnc-000649对PRRSV增殖具有抑制作用,但是其具体作用机制还有待进一步研究。
Long noncoding RNA(lncRNA)plays a role in immunity and the host response to viral infection.In this study,a novel porcine lncRNA lnc-000649,with a total length of 1483 bp,was found in porcine alveolar macrophage(PAM)cells.The expression level of lnc-000649 in PAM cells before and after PRRSV infection was detected by quantitative PCR,and it was found that the expression of lnc-000649 was significantly(P<0.01)down-regulated after PRRSV infection.In order to further study the function of this lncRNA,lnc-000649 overexpression vector lnc-000649-pcDNA3.1 was constructed and transfected into PAM cells.After PRRSV inoculation,the results showed that PRRSV RNA level in PAM cells which transfected with lnc-000649-pcdna3.1 was significantly(P<0.01)lower than that in transfected pcDNA3.1(+)and non-transfected group.It indicated that lnc-000649 could inhibit the proliferation of PRRSV.However,its specific mechanism remains to be further studied.
作者
吴俊静
乔木
周佳伟
梅书棋
彭先文
WU Junjing;QIAO Mu;ZHOU Jiawei;MEI Shuqi;PENG Xianwen(Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Science,Wuhan 430064,China)
出处
《浙江农业学报》
CSCD
北大核心
2021年第2期223-229,共7页
Acta Agriculturae Zhejiangensis
基金
湖北省重大科技创新项目(2019ABA084,2017ABA134)
湖北省创新群体项目(2018CFA014)
湖北省农业科技创新中心创新团队项目(2019-620-000-001-18)
国家生猪产业技术体系项目(CARS-36)。
