从ADAM33基因T2位点多态性分析小儿支气管哮喘易感性及其严重程度的关系

Analysis of susceptibility of bronchial asthma and its relationship with severity of bronchial asthma in children based on T2 locus polymorphism of ADAM33 gene

目的探讨整合素-金属蛋白酶33(ADAM33)基因T2位点多态性分析小儿支气管哮喘易感性及其严重程度。方法选择2017年1月至2019年3月本院收诊的支气管哮喘患儿47例为观察组,同期选取健康体检儿童47例为对照组。观察组中重度哮喘15例,中度17例,轻度15例。两组均行限制性片段长度多态性分析(聚合酶链式反应),比较两组平衡定律(Hardy-Weinberg)、T2位点等位基因频率,观察哮喘患儿严重程度与其T2位点多态性有无相关性。结果T2位点基因频率上,两组研究对象均有较好的群体代表性,均满足平衡定律。T2位点等位基因中,观察组A的基因频率高于对照组,差异有统计学意义(P<0.05),其中罹患支气管哮喘的高危因素是A等位基因增加。T2位点等位基因中,重度和中度哮喘患儿AG基因型频率均高于轻度哮喘患儿,差异有统计学意义(P<0.05),提示气管哮喘是否严重的高危因素是AG等位基因增加。结论支气管哮喘与ADAM33基因T2位点多态性存在相关性,一旦A的基因频率增加,其支气管哮喘罹患风险亦会相应增大,另外还能够根据AG等位基因增加情况判断支气管哮喘的严重程度。

Objective To investigate the T2 locus polymorphism of A Disintegrin and metalloproteinase 33(ADAM33)gene in the analysis of the susceptibility and severity of bronchial asthma(BA)in children.Methods Forty-seven child patients with BA admitted to the hospital from January 2017 to March 2019 were selected as the study group,and 47 healthy children who had physical examination during the same period were selected as the control group.There were 15 cases of severe asthma,17 moderate and 15 mild cases in the study group.All patients were subjected to restriction fragment length polymorphism analysis(polymerase chain reaction).The Hardy-Weinberg equilibrium and allele frequency of T2 locus were compared between the two groups.The correlation between BA severity and T2 locus polymorphism was observed.Results In regard to the gene frequency of the T2 locus,the subjects of the two groups had good group representation and all met the Hardy-Weinberg equilibrium.Among alleles of the T2 locus,the gene frequency of A of the study group was higher than that of the control group(P<0.05),and the high-risk factor for BA was the increase of the A allele.Among the T2 locus alleles,the AG genotype frequency of the children with severe or moderate asthma was higher than that of the children with mild asthma(P<0.05).The high-risk factor to distinguish the BA severity was the increase of AG allele.Conclusion There is a correlation between BA and T2 locus polymorphism of ADAM33 gene.Once the A gene frequency increases,the BA risk will increase accordingly.In addition,the severity of BA can be determined according to the increase of AG allele.