猪肉、牛肉中5种β-受体激动剂残留的LC-MS/MS分析方法的建立

LC-MS/MS Method Establishment for Five Kinds ofβ-Receptor Agonists Residues in Pork and Beef

【目的】本文采用超高效液相色谱-串联质谱法对猪肉和牛肉中5种β-受体激动剂残留含量进行检测。旨在建立一种快速有效的检测方法。【方法】样品经粉碎后用乙酸铵作为缓冲液,用氯化钠盐析,氨水乙腈和乙酸乙腈提取,提取液利用QuEChERS吸附剂净化后,用液相色谱-质谱/质谱仪测定,内标法定量。【结果】5种β-受体激动剂在0.5~100 ng/mL的浓度范围内相关系r2≥0.9998;检出限(LOD)为0.03~0.09μg/kg,定量限(LOQ)为0.10~0.30μg/kg;在低、中、高3种不同浓度水平的平均回收率在82.0%~113%之间,RSD值在0.23%~5.70%之间。【结论】此方法快速高效,可供相关检测人员参考。

[Objective]The ultra-performance liquid chromatography/tandem mass spectrometry(UPLC/MS/MS)method was used to detect 5 kinds ofβ-receptor agonists residues in pork and beef,aiming to establish a fast and effective detection method.[Method]After crushing the samples,ammonium acetate was used as the buffer.The extracting solution was obtained by salting-out with sodium chloride,ammonia-acetonitrile and acetonitrile-acetic acid.It was purified by QuEChERS adsorbents.It was measured by LC-MSMS.The internal standard was used for quantification.[Result]r2≥0.9998 within the concentration range of 0.5~100 ng/mL.The limit of detection(LOD)was 0.03~0.09μg/kg.The limit of quantitation(LOQ)was 0.10~0.30μg/kg.The average recovery rate was between 82.0%and 113%under low,medium and high concentration level.The value of RSD was between 0.23%and 5.7%.[Conclusion]The method is proved to be fast and effective and can provide references for related detection members.