运输应激对马血细胞和血液生化指标的影响

Effect of Transportation Stress on Horse Blood Cells and Blood Biochemical Indexes

为了调查运输应激对伊犁马血细胞和血液生化指标的影响,试验选取6匹伊犁母马,进行400 km里程的运输,总运输时间为8 h,分别在运输0、4、8 h和落地12 h后,测定马血清急性期蛋白(APPs)、皮质醇(Cor)、热休克蛋白(HSPs)、干扰素-γ(IFN-γ)和肿瘤坏死因子-α(TNF-α)含量以及血液常规指标。结果显示,与运输0 h相比,运输8 h马触珠蛋白(HP)含量显著上升(P<0.05),运输4、8 h和落地12 h后马血清Cor含量均极显著上升(P<0.01);落地12 h后马血清HSP90含量极显著下降(P<0.01);运输对马血清IFN-γ和TNF-α含量均无显著影响(P>0.05);运输4、8 h和落地12 h后血液红细胞分布宽度标准差(RDW-SD)、血小板分布宽度(PDW)、血小板大细胞比率(P-LCR)、粒细胞(GRAN)和粒细胞比率(GRAN%)均显著上升(P<0.05),中间细胞比率(MID%)、中间细胞(MID)、血红蛋白浓度(MCHC)均显著下降(P<0.05)。综上所述,运输应激对马血清HP、Cor、HSP90浓度、血液RDW-SD、PDW、P-LCR、GRAN%、GRAN、MID%、MID和MCHC均产生显著影响,这些指标可用于评价马对运输应激的敏感性。

In order to investigate the impact of transportation stress on blood cells and blood biochemical indicators of Ili horses,6 Ili mares were selected for transportation with a mileage of 400 km.The total transportation time were 8 h,the horse serum acute phase proteins(APPs),cortisol(Cor),heat shock proteins(HSPs),interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α)and blood routine were detected at 0,4,8 h of transport and 12 h after landing.The results showed that when compared with 0 h of transport,horses serum haptoglobin(HP)content increased significantly at 8 h of transport(P<0.05),horses serum Cor content increased significantly at 4 and 8 h of transport and 12 h after transport(P<0.01).Horses serum HSP90 content decreased significantly at the 12 h after landing(P<0.01),but transportation had no significant effect on serum IFN-γand TNF-αcontent(P>0.05).The standard deviation in red cell distribution width(RDW-SD),platelet distribution width(PDW),platelet large cell ratio(P-LCR),granulocyte(GRAN)and granulocyte ratio(GRAN%)were all significantly increased at 4 and 8 h of transport and 12 h after transport(P<0.05),and intermediate cells ratio(MID%),intermediate cells(MID),and hemoglobin concentration(MCHC)were all significantly decreased at 4 and 8 h of transport and 12 h after transport(P<0.05).In conclusion,transportation stress had significant effects on horses serum HP,Cor,HSP90 concentration,blood RDW-SD,PDW,P-LCR,GRAN%,GRAN,MID and MCHC,these indicators could be used to evaluate horses sensitivity to transportation stress.