NE-CQDs/荧光素双发射比率荧光探针测定吲达帕胺的研究

NE-carbon dots/fluorescein based dual-emission ratiometric probe for indapamide detection

以天然物质油桃为唯一碳源,一步水热法制得荧光性能良好并呈蓝紫色荧光的油桃碳量子点(NE-CQDs),对NE-CQDs形貌和发光性能进行了表征。在pH=1.70的氯化钾-盐酸缓冲溶液中,将NE-CQDs与荧光素复合,在λex=320 nm单一波长激发下,NE-CQDs/荧光素复合物于395 nm/510 nm两处同时产生荧光发射峰;在该体系中加入Ce(Ⅳ),λem/λem=395 nm/510 nm两波长处的荧光信号均发生明显猝灭;吲达帕胺(IDP)的加入仅可使λem=510 nm处的荧光强度恢复,而λem=395 nm处荧光信号基本不变,由此可构建基于NE-CQDs/荧光素双发射荧光比率探针测定IDP的方法。实验探讨了Ce(Ⅳ)对NE-CQDs/荧光素探针的荧光猝灭机理。在优化的实验条件下,8.0×10^(-7) mol·L^(-1)~1.0×10^(-4) mol·L^(-1)范围内的IDP与NE-CQDs/荧光素探针于510 nm/395 nm两处荧光强度比值呈良好的线性关系,检出限(3σ/k)为1.06×10^(-7) mol·L^(-1)。该探针用于样品中IDP含量的测定,结果满意。

Using the fresh nectarine as the only carbon source,a new nectarine carbon quantum dots(NE-CQDs)with good fluorescent properties,showing blue-violet fluorescence,were synthesized by hydrothermal method.The morphology and luminescence properties of NE-CQDs were characterized.After combining NE-CQDs with fluorescein in KCl-HCl buffer(pH=1.70),the NE-CQDs/fluorescein probe shows two fluorescence emission peaks at 395 nm/510 nm at single wavelength excitation of 320 nm.While both peaks at 395 and 510 nm were quenched after Ce(Ⅳ)was added into the system.The fluorescence signal atλem=510 nm was restored with the additional of indapamide(IDP),where as the fluorescence signal atλem=395 nm was unchanged.Thus,a new method for the detection of IDP based on NE-CQDs/fluorescein fluorescence ratiometric probe was established,to further extend,the quenching mechanism of Ce(Ⅳ)was explored.Under the optimized experimental conditions,there is a good linear relationship between the concentration of IDP and the fluorescence intensity ratio of NE-CQDs/fluorescein probe atλem/λem=510 nm/395nm,with a linear range of 8.0×10^(-7) mol·L^(-1)~1.0×10^(-4) mol·L^(-1).The detection limit was calculated to be 1.06×10^(-7)(3σ/k).This probe was used in the detection of the content of IDP in the samples and showing satisfactory results.