模型动物神经元钙信号检测技术研究与进展

Research and development of in vivo detection technology for calcium signals in model animal neurons

钙是模型动物神经细胞内重要的第二信使,参与细胞多种功能的调节,可以产生多种细胞内信号,这些信号在神经元功能调控及信息传递方面发挥着重要作用。在明确的细胞亚区,钙信号发挥着高度特异性的功能,尤其在大脑皮层中更能反映神经元的活性,因此神经元钙信号的检测对研究大脑皮层功能至关重要。本文综述了模型动物钙信号检测的方法,目前比较常见的方法包括双光子钙成像技术、深脑显微钙成像技术、光纤记录技术。通过文献回顾分析,了解了该领域的新进展,并综述了各种钙信号在体检测技术在模型动物神经元信号分析和神经元可塑性研究的应用及前景。

Calcium is an important messenger in mammalian nerve cells.It mediates a variety of intracellular signal transduction pathways and plays an important role in regulating neuronal functions.Calcium signaling performs its highly specific functions in a defined subregion of the cell,especially in the cortex.Detection of calcium signals in neurons is particularly important to study neuronal functions.In this article,we review the method of detecting calcium signals in model animals.The common method include two-photon calcium imaging technology,deep brain microscopic calcium imaging technology,and optical fiber recording technology.Through a literature review and analysis,new developments in this field are presented.We also discuss the prospects of calcium signal detection to study cortical neuronal signals and plasticity in various animal models.