血清IL-35与抗PPD抗体在活动性肺结核中的诊断价值

Diagnostic values of serum IL-35 and anti-PPD antibody in active pulmonary tuberculosis

目的探究血清白细胞介素-35(IL-35)与抗结核菌素的纯蛋白衍化物(PPD)抗体在活动性肺结核中的诊断价值。方法选择94例活动性肺结核患者为研究组,88例同期到本院体检者为对照组。测定两组血清IL-35、外周血IL-35亚基mRNA水平,抗PPD-IgG浓度,分析各指标对活动性肺结核诊断价值。研究组患者依据病情严重程度进行分组,比较不同病情严重程度患者外周血指标水平,分析血清IL-35及其亚基mRNA水平与抗PPD-IgG相关性。结果研究组患者血清IL-35、外周血EBI3 mRNA、P35 mRNA以及血清抗PPD-IgG水平明显高于对照组(t分别=19.18、16.14、21.24、12.16,P均<0.05);ROC曲线显示,血清IL-35、外周血EBI3 mRNA、P35 m RNA、血清抗PPD-IgG以及各指标联合诊断活动性肺结核曲线下面积分别为0.71、0.67、0.63、0.85、0.87。研究组血清IL-35、外周血EBI3 mRNA、P35 mRNA以及血清抗PPD-IgG水平与病情严重程度有关(F分别=15.98、14.23、7.77、20.70,P均<0.05);Pearson相关性分析显示,研究组血清IL-35与抗PPD-IgG水平呈正相关(r=0.64,P<0.05)。结论活动性肺结核患者血清IL-35水平异常上升,与抗PPD抗体以及病情严重程度关系密切。

Objective To explore the diagnostic values of serum interleukin-35(IL-35)and anti-protein purified derivative(PPD)antibody in active pulmonary tuberculosis.Methods Totally 94 patients with active pulmonary tuberculosis were selected as the study group,and 88 healthy people who receiving physical examination during the same period were enrolled as the control group.Serum IL-35 level,peripheral blood IL-35 subunit mRNA level,and anti-PPDIgG concentration in both groups were measured and compared.The diagnostic values of those parameters in active pulmonary tuberculosis were analyzed.Patients in the study groups were grouped into three groups according to the severity of the disease,and the serum IL-35,peripheral blood EBI3 mRNA,P35 mRNA,and serum anti-PPD-IgG levels among them were compared.The correlation between serum IL-35 and anti-PPD-IgG was analyzed.Results Serum IL-35,peripheral blood EBI3 mRNA,P35 mRNA and serum anti-PPD-IgG levels in the study group were significantly higher than those in the control group(t=19.18,16.14,21.24,12.16,P<0.05).ROC curves showed that AUC values of serum IL-35,peripheral blood EBI3 mRNA,P35 mRNA,serum anti-PPD-IgG level and combination of the indexes in diagnosis of active pulmonary tuberculosis were 0.71,0.67,0.63,0.85,and 0.87,respectively.Serum IL-35,peripheral blood EBI3 mRNA,P35 mRNA and serum anti-PPD-IgG levels were the lowest in mild group,followed by moderate group,and the highest in severe group(F=15.98,14.23,7.77,20.70,P<0.05).Pearson correlation analysis showed that serum IL-35 was positively correlated with anti-PPD-IgG level(r=0.64,P<0.05).Conclusion Serum IL-35 level is abnormally elevated in patients with active pulmonary tuberculosis,which is closely related to anti-PPD antibody and the severity of the disease.