QuEChERS-UPLC-MS/MS同时测定玉米田环境中氯氟吡氧乙酸和氯氟吡氧乙酸异辛酯的残留量

Simultaneous Determination of Residues of Fluroxypyr and Fluroxypr Mepthyl in Maize Field by QuEChERS-UPLC-MS/MS

建立了使用分散固相萃取的QuEChERS结合超高效液相色谱串联质谱(UPLC-MS/MS)对玉米、鲜食玉米、秸秆以及土壤中氯氟吡氧乙酸和氯氟吡氧乙酸异辛酯进行定量测定的方法。结果表明,氯氟吡氧乙酸和氯氟吡氧乙酸异辛酯在0.005~0.5 mg/L内线性关系良好,相关系数高于0.9998。氯氟吡氧乙酸异辛酯和氯氟吡氧乙酸在玉米、鲜食玉米、秸秆和土壤中的平均添加回收率在84.6%~103.8%,相对标准偏差(RSD)为0.7%~5.4%。氯氟吡氧乙酸和氯氟吡氧乙酸异辛酯在玉米、鲜食玉米、秸秆以及土壤中的定量限(LOQ)均为0.05 mg/kg,最低检测限(LOD)分别为1.1×10^(-3) ng和2.5×10^(-3) ng。该方法准确度、精密度和灵敏度较高,线性关系良好,具有简便、快速、准确及分离效果好的优点。

A QuEChERS combined with UPLC-MS/MS was established for simultaneous determination of fluroxypyr and fluroxypyr mepthyl in maize,fresh corn,straw and soil.The results showed that the linear relationship of fluroxypyr and fluroxypyr mepthyl was good in the range of 0.005-0.5 mg/L,and the correlation coefficient was higher than 0.9998.The average addition recovery rates of fluroxypyr and fluroxypyr mepthyl in corn,fresh corn,straw,and soil ranged from 84.6% to 103.8%,with a relative standard deviation of 0.7%-5.4%.The limit of quanlification of fluroxypyr and fluroxypyr mepthyl in corn,fresh corn,straw and soil were 0.05 mg/kg.The lowest detectable condentrations were 1.1×10^(-3) ng and 2.5×10^(-3) ng,respectively.This method has high accuracy,precision and sensitivity,good linear relationship,and has the advantages of simplicity,fastness,accuracy,and good separation effect.