先兆子痫患者胎盘中Lumican及自噬相关蛋白水平的表达变化

Expression changes of Lumican and autophagy-related proteins in placenta of patients with preeclampsia

目的观察先兆子痫(PE)患者胎盘中Lumican及自噬相关蛋白水平的表达变化。方法收集2019年1月至12月就诊于日照市人民医院产科19例PE患者的胎盘标本和21例正常人的胎盘标本,分别记为PE组和对照组。实时荧光定量聚合酶链反应(RT-qPCR)和蛋白质免疫印迹(Western blotting)检测胎盘组织中Lumican的表达水平;免疫组织化学染色观察胎盘组织中自噬标记蛋白微管相关蛋白轻链3(LC3B)和Beclin1的表达水平;检测胎盘组织超氧化物歧化酶(SOD)的活力和丙二醛(MDA)的水平,分析细胞氧化应激水平。结果在PE组和对照组胎盘组织中均有Lumican mRNA和蛋白的表达,且PE组Lumican mRNA(1.04±0.11)及蛋白(0.17±0.02)的相对表达量均低于正常对照组mRNA(2.81±0.11,t=25.52,P<0.01)和蛋白(0.98±0.15,t=12.07,P<0.01)。PE组Beclin1和LC3B的表达均低于正常对照组。PE组SOD的活力值[(201.31±26.11)U/mgprot]低于正常对照组[(241.65±25.28)U/mgprot],而MDA的水平[(6.70±1.10)nmol/mgprot]高于正常对照组[(5.10±1.05)nmol/mgprot],均差异有统计学意义(t=3.50,3.33;均P<0.01)。结论人胎盘组织中存在Lumican的表达,PE患者胎盘组织中Lumican的表达较正常胎盘组织明显下降,氧化应激水平升高,同时自噬相关蛋白表达水平下降。

Objective To investigate the expression changes of Lumican and autophagy-related proteins in placenta of patients with preeclampsia.Methods The placental specimens from 19 patients with preeclampsia and 21 healthy puerpera in the Obstetrics Department of People′s Hospital of Rizhao from January to December 2019 were collected.RT-qPCR and Western blotting were used to detect the expression of Lumican in human placenta.The expression levels of autophagy marker proteins microtubule-associated protein light chain 3(LC3B)and Beclin1 in placental tissues were observed by immunohistochemical staining.The vitality of superoxide dismutase(SOD)and level of malondialdehyde(MDA)in placenta tissues were detected to analyse the level of oxidative stress.Results The expressions of Lumican mRNA(1.04±0.11)and protein(0.17±0.02)in PE group were lower than those in control group(2.81±0.11,0.98±0.15,t=25.52,12.07,all P<0.01).Immunohistochemical results showed that the expressions of Beclin1 and LC3B in PE group were lower than those in control group.The vitality of SOD in PE group[(201.31±26.11)U/mgprot]remarkablely decreased compared with that in control group[(241.65±25.28)U/mgprot](t=3.50,P<0.01),while the level of MDA in PE group[(6.70±1.10)nmol/mgprot]remarkablely increased compared with that in control group[(5.10±1.05)nmol/mgprot](t=3.33,P<0.01).Conclusion There is higher Lumican expression in human placental tissue,but the expression level of Lumican in placental tissue significantly decreases in PE,meanwhile it shows a higher level of oxidative stress,and decreased expression of autophagy related proteins.