西南区域核心地区中老年男性自然人群饮酒量与口腔菌群关系初探

A preliminary study on the relationship between alcohol consumption and oral flora of middle-aged and elderly men from the core area of southwestern China

目的分析西南区域核心地区中老年男性自然人群饮酒量对口腔菌群的影响,探寻超量饮酒相关菌群与饮酒相关癌症的关系。方法2018年3月-6月,采集目标人群唾液样本进行16S核糖体RNA基因测序,并进行以饮酒史为目标变量的问卷调查。根据饮酒量将受试者分为不饮酒组、适量饮酒组、超量饮酒组,进行菌群α多样性分析、组间菌群丰富度差异分析、菌群功能预测、受试者操作特征(receiver operating characteristic,ROC)曲线模型预测等分析。结果共纳入59例调查对象,其中不饮酒组23例(39.0%),适量饮酒组23例(39.0%),超量饮酒组13例(22.0%);平均年龄为(61.90±8.85)岁。超量饮酒可提高口腔菌群丰富度(P<0.05),改变消化链球菌(Peptostreptococcus)、TM7门[G-6]菌(TM7_[G-6])等特定菌属丰度(P<0.05),调节癌症相关通路(P<0.05)。ROC曲线分析发现TM7_[G-6]等3种菌属可有效区分不饮酒组与超量饮酒组(曲线下面积=0.915)。结论消化链球菌(Peptostreptococcus)、TM7门[G-6]菌(TM7_[G-6])菌属为西南区域核心地区中老年男性自然人群潜在超量饮酒标志物菌群,超量饮酒相关菌群与口腔癌关系密切。

Objective To analyze the effects of alcohol consumption on oral flora of middle-aged and elderly men from the core area of southwestern China,and explore the relationship between excessive-alcohol-consumptionrelated flora and alcohol-related cancer.Methods From March to June 2018,saliva samples of target subjects were collected for 16S ribosomal RNA gene sequencing,and a questionnaire survey which took drinking history of each participant as the target variable was conducted.According to the amount of alcohol consumed,the subjects were divided into non-drinking group,moderate-drinking group,and excessive-drinking group.The microbial analysis ofαdiversity,analysis of group difference of oral flora abundance,bacterial function prediction,and receiver operating characteristic(ROC)curve model prediction were carried out.Results A total of 59 subjects were included.There were 23 cases(39.0%)in the non-drinking group,23 cases(39.0%)in the moderate-drinking group,and 13 cases(22.0%)in the excessive-drinking group.The average age was(61.90±8.85)years.Excessive drinking increased the abundance of oral flora(P<0.05),and could change the abundance of specific genus such as Peptostreptococcus and TM7[G-6](P<0.05)and regulate cancer-related pathways(P<0.05).ROC analysis found that a panel of three genus oral bacteria such as TM7[G-6]might effectively distinguish the non-drinking group from the excessive-drinking group(area under curve=0.915).Conclusions Genus of Peptostreptococcus and TM7_[G-6]are the potential oral flora biomarkers for the excessivedrinking of target subjects.Some excessive drinking-related flora are closely related to oral cancer.