畜禽产品中四环素类药物残留色谱质谱检测技术及前处理方法研究进展

Research Progress on the Detection of Tetracycline Residues in Livestock and Poultry Products by LC-MS and Study on Pre-treatment Methods

四环素类药物是一类广谱抗菌药物,在畜牧业中得到广泛应用。该药物的不合理使用甚至滥用,导致环境及动物源性食品中此类药物残留超标,从而威胁公共卫生安全。液相色谱法及液相色谱串联质谱法是检测畜禽产品中四环素类药物残留的主要方法。这类方法的难点及关键步骤是样品的前处理,也是目前研究的热点。本文就目前动物源性食品中四环素类药物残留色谱及质谱分析法,特别是样品前处理方法的研究进展进行综述。近年来新净化材料、新提取剂及新提取方法不断出现,提升了提取及净化效果,减少了试剂用量,提高了工作效率,并能够同时实现多类药物的提取和检测,有力保障了食品和环境安全。

Tetracyclines,a class of broad-spectrum antibiotics,has been widely applied in animal husbandry.However,their residues in environment and animal-derived food would exceed the standard in the event of any unreasonable or abuse use,thus pose a threat to public health safety.Liquid chromatography(LC)and liquid chromatography-mass spectrometry(LC-MS)were the main methods to detect tetracycline residues in livestock and poultry products,and their difficulty and key step was pre-treatment of samples that was also a hotspot under study.In the paper,the LC and LC-MS for detection of tetracycline residues in animal-derived food,especially the research progress of sample pre-treatment methods,were summarized.With the emerging new purification materials,extraction agents and methods,the safety of food and environment was strongly safeguarded through improving the effectiveness of extraction and purification,reducing the dosage of reagents,increasing work efficiency as well as simultaneously extracting and detecting multiple drugs.